一株沼泽红假单胞菌 GJ-22 所分泌胞外多糖的结构特征与功能活性。
Structural characterization and functional activity of an exopolysaccharide secreted by Rhodopseudomonas palustris GJ-22.
机构信息
Longping Branch, Graduate School of Hunan University, Changsha 410125, China.
Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
出版信息
Int J Biol Macromol. 2021 Jan 15;167:160-168. doi: 10.1016/j.ijbiomac.2020.11.165. Epub 2020 Nov 27.
One water exopolysaccharide, designated G-EPS, was secreted by Rhodopseudomonas palustris GJ-22 culture media. The structure of G-EPS was characterized with HPGPC, GC-MS, methylation, 1D and 2D NMR, along with UV and FT-IR spectrum. The G-EPS molecular weight was 10.026 kilodalton, and is composed of D-mannose (92.8%) and d-glucose (7.2%). The purified G-EPS promoted plant growth and induced systemic resistance against TMV in Nicotiana benthamiana. These results suggested that G-EPS is an important active component of the bio-control capacity of GJ-22.
一株沼泽红假单胞菌（Rhodopseudomonas palustris GJ-22）发酵液中分泌出一种胞外多糖，命名为 G-EPS。采用高效凝胶渗透色谱（HPGPC）、气相色谱-质谱联用（GC-MS）、甲基化、一维和二维核磁共振（1D 和 2D NMR）以及紫外和傅里叶变换红外光谱（UV 和 FT-IR）等方法对 G-EPS 的结构进行了表征。G-EPS 的分子量为 10.026 千道尔顿，由 D-甘露糖（92.8%）和 d-葡萄糖（7.2%）组成。纯化的 G-EPS 可促进烟草原生质体生长，并诱导烟草原生质体对 TMV 产生系统抗性。这些结果表明 G-EPS 是 GJ-22 生防能力的重要活性成分。
Zotero 插件