Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.
Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.
Microb Pathog. 2021 Jan;150:104659. doi: 10.1016/j.micpath.2020.104659. Epub 2020 Nov 27.
Enteroviruses (Picornaviridae) and astroviruses (Astroviridae) cause various diseases in humans and animals, including in non-human primates (NHPs). Some enteroviruses and astroviruses detected in NHPs are genetically related to those infecting humans, indicating the occurrence of interspecies transmissions. In this study, we screened 200 fecal samples of 56 free-ranging mandrills (Mandrillus sphinx) by nested reverse transcription-PCR with primers targeting the VP1 and RdRp genes, to evaluate the diversity of enterovirus and astrovirus infection, respectively, and the associated zoonotic risk. Overall, ten samples from six mandrills were enterovirus-positive (5%), and three samples from three mandrills were astrovirus-positive (1.5%). This is the first evidence of astrovirus infection in mandrills. Phylogenetic analyses based on the VP1 sequences revealed that all ten enterovirus sequences were part of the species Enterovirus J, suggesting low zoonotic risk. Phylogenetic analysis of the three astrovirus sequences showed that they all belonged to the Mamastrovirus genus. Two astrovirus sequences were highly divergent from all human astrovirus sequences (63.4-73% nucleotide identity), while one sequence (AstV-5) suggested cross-species transmission from humans to mandrills. Additional studies are needed to better characterize the identified astroviruses and to confirm whether mandrills are host of astroviruses than can be transmitted to humans.
肠道病毒（小 RNA 病毒科）和星状病毒（星状病毒科）可引起人类和动物的各种疾病，包括非人类灵长类动物（NHPs）。在 NHPs 中检测到的一些肠道病毒和星状病毒与感染人类的病毒在基因上有关，表明存在种间传播。在这项研究中，我们通过针对 VP1 和 RdRp 基因的巢式逆转录-PCR 对 56 只自由放养的山魈（Mandrillus sphinx）的 200 份粪便样本进行了筛选，以分别评估肠道病毒和星状病毒感染的多样性，以及相关的人畜共患病风险。总的来说，6 只山魈的 10 份样本（5%）呈肠道病毒阳性，3 只山魈的 3 份样本（1.5%）呈星状病毒阳性。这是山魈感染星状病毒的首例证据。基于 VP1 序列的系统发育分析显示，所有 10 个肠道病毒序列均属于肠道病毒 J 种，表明人畜共患病风险较低。对 3 个星状病毒序列的系统发育分析表明，它们均属于 Mamastrovirus 属。2 个星状病毒序列与所有人类星状病毒序列的核苷酸同一性差异较大（63.4-73%），而一个序列（AstV-5）表明存在从人类到山魈的跨种传播。需要进一步的研究来更好地描述鉴定出的星状病毒，并确认山魈是否为可传播给人类的星状病毒宿主。
