Bohou Kombila Linda, N'dilimabaka Nadine, Garcia Déborah, Rieu Océane, Engone Ondo Jéordy Dimitri, Ndong Mebaley Telstar, Boundenga Larson, Fritz Matthieu, Lenguiya Léadisaelle Hosanna, Maganga Gael Darren, Leroy Eric M, Becquart Pierre, Mombo Illich Manfred
Unité Émergence des Maladies Virales (UEMV), Département de Virologie, Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville BP 769, Gabon.
Département de Biologie, Université des Sciences et Techniques de Masuku (USTM), Franceville BP 941, Gabon.
Animals (Basel). 2023 Aug 3;13(15):2512. doi: 10.3390/ani13152512.
Astroviruses (AstVs) enteroviruses (EVs), and caliciviruses (CaVs) infect several vertebrate taxa. Transmitted through the fecal-oral route, these enteric viruses are highly resistant and can survive in the environment, thereby increasing their zoonotic potential. Here, we screened for AstVs, EVs, and CaVs to investigate the role of domestic animals in the emergence of zoonoses, because they are situated at the human/wildlife interface, particularly in rural forested areas in Central Africa. Rectal swabs were obtained from 123 goats, 41 sheep, and 76 dogs in 10 villages located in northeastern Gabon. Extracted RNA reverse-transcribed into cDNA was used to detect AstVs, EVs, and CaVs by amplification of the RNA-dependent RNA polymerase (RdRp), or capsid protein (VP1) gene using PCR. A total of 23 samples tested positive, including 17 goats for AstVs, 2 goats, 2 sheep, 1 dog for EVs, and 1 dog for CaVs. Phylogenetic analyses revealed that AstV RdRp sequences clustered with sheep-, goat-, or bovine-related AstVs. In addition, one goat and two sheep VP1 sequences clustered with caprine/ovine-related Evs within the species, and the CaV was a canine vesivirus. However, human-pathogenic Evs, EV-B80 and EV-C99, were detected in goats and dogs, raising questions on the maintenance of viruses able to infect humans.
星状病毒（AstVs）、肠道病毒（EVs）和杯状病毒（CaVs）可感染多种脊椎动物类群。这些肠道病毒通过粪口途径传播，具有高度抗性，能在环境中存活，从而增加了其人畜共患病传播的可能性。在此，我们对星状病毒、肠道病毒和杯状病毒进行筛查，以研究家畜在人畜共患病出现过程中所起的作用，因为家畜处于人类/野生动物的交叉区域，尤其是在中非的农村森林地区。我们从加蓬东北部10个村庄的123只山羊、41只绵羊和76只狗身上采集了直肠拭子。提取的RNA逆转录成cDNA后，通过使用PCR扩增RNA依赖性RNA聚合酶（RdRp）或衣壳蛋白（VP1）基因来检测星状病毒、肠道病毒和杯状病毒。共有23个样本检测呈阳性，其中17只山羊感染星状病毒，2只山羊、2只绵羊、1只狗感染肠道病毒，1只狗感染杯状病毒。系统发育分析表明，星状病毒RdRp序列与绵羊、山羊或牛相关的星状病毒聚类。此外，一只山羊和两只绵羊的VP1序列在该物种内与山羊/绵羊相关的肠道病毒聚类，而杯状病毒是一种犬水疱病毒。然而，在山羊和狗身上检测到了人类致病性肠道病毒EV - B80和EV - C99，这引发了关于能够感染人类的病毒如何存续的问题。
