College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, 518071, PR China.
Fujian Academy of Agricultural Sciences Fuzhou, 350002, Fujian, PR China.
Biochimie. 2021 Jan;180:68-78. doi: 10.1016/j.biochi.2020.10.008. Epub 2020 Oct 24.
A valuable plant, Cyclobalanopsis gilva, (C. gilva) has a low germination rate (below 50%) under its natural habitations. In order to examine the reasons for the low germination rate, the seeds of C. gilva (germinated and non-germinated) were evaluated using comparative proteomics analysis. A total of 3078 differentially abundant proteins (DAPs) were identified through a label-free method; most DAPs up-accumulated in germinated seeds were related to carbohydrates metabolism. Furthermore the proteins related to the signals, stress, and protein metabolism showed up-accumulation in germinated and no abundance or down-accumulation in non-germinated seeds. Enzyme activity of HK, PGK, PFK, and PK from glycolysis in SG-Control samples were 1.7-, 1.1-, 1.4-, and 1.3-times higher compared with those in control ones while CS, NAD-MDH, α-KGDH, and ICDH from the TCA cycle in SG-Control samples were 3, 1.1, 1.2, and 1.2 times higher than those in NG-Control ones. The β-amylase activity was 4-fold higher in successfully germinated seeds compared to non-germinated seeds. Interestingly, α-amylase did not show significant changes in protein abundance and enzyme activity among the three samples. The present findings reveal that unsuccessful germination of C. gilva seeds is due to lack of energy.
一种有价值的植物，木荷（Cyclobalanopsis gilva），在其自然栖息地的发芽率（低于 50%）很低。为了研究发芽率低的原因，使用比较蛋白质组学分析评估了木荷的种子（已发芽和未发芽）。通过无标记方法鉴定了总共 3078 种差异丰度蛋白（DAP）；大多数在发芽种子中积累的 DAP 与碳水化合物代谢有关。此外，与信号、应激和蛋白质代谢相关的蛋白质在发芽种子中积累，而在未发芽种子中则没有丰度或减少。SG-Control 样品中糖酵解途径的 HK、PGK、PFK 和 PK 的酶活性分别比对照样品高 1.7、1.1、1.4 和 1.3 倍，而 SG-Control 样品中 TCA 循环的 CS、NAD-MDH、α-KGDH 和 ICDH 的酶活性分别比 NG-Control 样品高 3、1.1、1.2 和 1.2 倍。与未发芽种子相比，成功发芽的种子中的β-淀粉酶活性高 4 倍。有趣的是，α-淀粉酶在三个样品中的蛋白丰度和酶活性上没有明显变化。本研究结果表明，木荷种子的发芽失败是由于缺乏能量。
