Zaynab Madiha, Sharif Yasir, Xu Zhaoshi, Fiaz Sajid, Al-Yahyai Rashid, Yadikar Hamad A, Al Kashgry Najla Amin T, Qari Sameer H, Sadder Monther, Li Shuangfei
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Plants (Basel). 2023 Aug 11;12(16):2923. doi: 10.3390/plants12162923.
The gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illustrated that several genes were without introns. Furthermore, the subcellular localization results suggested that GmDUF668 proteins were present in the nucleus, mitochondria, cytoplasm, and plasma membrane. promoters were analyzed in silico to gain insight into the presence of regulatory sequences for TFs binding. The expression profiling illustrated that genes showed expression in leaves, roots, nodules, and flowers. To investigate their response to salt stress, we utilized the RNA sequencing data of genes. The results unveiled that , , and genes were upregulated against salt stress treatment. We further validated these findings using qRT-PCR analysis. These findings provide a scientific basis to explore the functions of genes against different stress conditions.
该基因在减轻非生物胁迫因素的影响方面发挥着关键作用。在本研究中，我们在大豆基因组中鉴定出30个基因，分布在15条染色体上。系统发育分析将这些基因分为三组（第一组、第二组和第三组）。有趣的是，基因结构分析表明，几个基因没有内含子。此外，亚细胞定位结果表明，GmDUF668蛋白存在于细胞核、线粒体、细胞质和质膜中。通过计算机分析启动子，以深入了解转录因子结合调控序列的存在情况。表达谱分析表明，这些基因在叶、根、根瘤和花中均有表达。为了研究它们对盐胁迫的反应，我们利用了这些基因的RNA测序数据。结果表明， 、 和 基因在盐胁迫处理下上调。我们进一步使用qRT-PCR分析验证了这些发现。这些发现为探索这些基因在不同胁迫条件下的功能提供了科学依据。
