Institute of Biological Information Processing, Molecular and Cellular Physiology, IBI-1, Forschungszentrum Jülich GmbH, Jülich, Germany.
Department of Ophthalmology, RWTH Aachen University, Aachen, Germany.
Invest Ophthalmol Vis Sci. 2020 Nov 2;61(13):37. doi: 10.1167/iovs.61.13.37.
In RP, photoreceptors degenerate. Retinal prostheses are considered a suitable strategy to restore vision. In animal models of RP, a pathologic rhythmic activity seems to compromise the efficiency of retinal ganglion cell stimulation by an electrical prosthesis. We, therefore, strove to eliminate this pathologic activity.
Electrophysiologic recordings of local field potentials and spike activity of retinal ganglion cells were obtained in vitro from retinae of wild-type and rd10 mice using multielectrode arrays. Retinae were stimulated electrically.
The efficiency of electrical stimulation was lower in rd10 retina than in wild-type retina and this was highly correlated with the presence of oscillations in retinal activity. Glycine and GABA, as well as the benzodiazepines diazepam, lorazepam, and flunitrazepam, abolished retinal oscillations and, most important, increased the efficiency of electrical stimulation to values similar to those in wild-type retina.
Treatment of patients with these benzodiazepines may offer a way to improve the performance of retinal implants in cases with poor implant proficiency. This study may open the way to a therapy that supports electrical stimulation by prostheses with pharmacologic treatment.
在 RP 中，光感受器会退化。视网膜假体被认为是恢复视力的一种合适策略。在 RP 的动物模型中，病理性节律活动似乎会降低电假体对视网膜神经节细胞刺激的效率。因此，我们努力消除这种病理性活动。
使用多电极阵列从野生型和 rd10 小鼠的视网膜中获得体外局部场电位和视网膜神经节细胞的尖峰活动的电生理记录。视网膜受到电刺激。
与野生型视网膜相比，rd10 视网膜的电刺激效率较低，这与视网膜活动中的振荡高度相关。甘氨酸和 GABA 以及苯并二氮䓬类药物地西泮、劳拉西泮和氟硝西泮消除了视网膜振荡，最重要的是，将电刺激的效率提高到与野生型视网膜相似的水平。
用这些苯并二氮䓬类药物治疗患者可能为改善植入效率差的患者的视网膜植入物的性能提供一种方法。这项研究可能为通过药物治疗支持假体电刺激的治疗方法开辟道路。
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