Jung Marie, Wang Jing, Montes Viviana Rincón, Offenhäusser Andreas, Willuweit Antje, Müller Frank
Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich, Jülich, Germany.
Institute of Neuroscience and Medicine (INM-4), Medical Imaging Physics, Forschungszentrum Jülich, Jülich, Germany.
PLoS One. 2025 May 27;20(5):e0324345. doi: 10.1371/journal.pone.0324345. eCollection 2025.
Retinitis pigmentosa (RP) is a leading cause of blindness. The best studied models of human RP are the rd1 and rd10 mouse and the RCS rat (Royal College of Surgeons). In many models after degeneration of the photoreceptors, a pathological rhythmic activity of the retina as well as lowered efficiency of electrical stimulation were observed. In rd10 retina, both events were shown to be intimately linked. Surprisingly, to our knowledge no retinal oscillations have been reported in RCS retina. As oscillations might interfere with the performance of therapeutic approaches to restore vision, e.g., retinal prostheses, it is important to know, whether they are a common feature of retinal degeneration. Electrical activity was recorded in retinae of 3-19 months (M3-19) old RCS rats in vitro using planar and penetrating multi-electrode-arrays. Short deflections in the local field potential resembling those observed in oscillations in rd1 and rd10 retinae were only sporadically found in M3 RCS retinae. Oscillations at appr. 2 Hz occurred more often and were more pronounced the older the animals were. Yet, even at M18-19 oscillatory periods were short and separated by long periods of non-oscillatory activity. In summary, in advanced stages of degeneration, RCS retinae display oscillations similar to rd1 and rd10 retinae. However, in RCS retina oscillatory periods are shorter than in mouse models and may, therefore, have escaped detection in earlier studies. These results together with results observed in non-rodent models suggest that pathological rhythmic activity is a common feature in RP models.
视网膜色素变性（RP）是导致失明的主要原因。研究最深入的人类RP模型是rd1和rd10小鼠以及RCS大鼠（皇家外科学院大鼠）。在许多模型中，光感受器退化后，观察到视网膜出现病理性节律活动以及电刺激效率降低。在rd10视网膜中，这两种情况被证明密切相关。令人惊讶的是，据我们所知，尚未有关于RCS视网膜中视网膜振荡的报道。由于振荡可能会干扰恢复视力的治疗方法（如视网膜假体）的性能，因此了解它们是否是视网膜退化的共同特征非常重要。使用平面和穿透式多电极阵列在体外记录3至19个月（M3 - 19）大的RCS大鼠视网膜的电活动。在M3 RCS视网膜中仅偶尔发现类似于在rd1和rd10视网膜振荡中观察到的局部场电位的短偏转。约2Hz的振荡出现得更频繁，并且动物年龄越大越明显。然而，即使在M18 - 19时，振荡周期也很短，并且被长时间的非振荡活动隔开。总之，在退化的晚期阶段，RCS视网膜表现出与rd1和rd10视网膜相似的振荡。然而，在RCS视网膜中，振荡周期比小鼠模型中的短，因此可能在早期研究中未被检测到。这些结果与在非啮齿动物模型中观察到的结果一起表明，病理性节律活动是RP模型中的一个共同特征。
