Jain Aditi, Behera Manisha, Mahapatra Chinmaya, Sundaresan Nagalingam R, Chatterjee Kaushik
Centre for BioSystems Science and Engineering, Indian Institute of Science, Bangalore 560012, India.
Department of Materials Engineering, Indian Institute of Science, Bangalore 560012, India.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111416. doi: 10.1016/j.msec.2020.111416. Epub 2020 Aug 24.
Reactive oxygen species (ROS) are generated in reperfused ischemic heart tissue after myocardial infarction (MI). A compensatory attempt of the heart to enhance its functional performance after MI is to undergo cardiomyocyte hypertrophy. In the past, reducing the levels of ROS in the cardiomyocytes has been linked to suppression of cardiac hypertrophy. Notably, cerium oxide nanoparticles (nCe) have been used extensively to protect the cells from oxidative damage by efficiently scavenging cellular ROS. Furthermore, fibrous matrices such as nanofibers are emerging as promising substrates for engineering implantable cardiac patches. In this study, we describe the fabrication of nCe-decorated polycaprolactone (PCL) and PCL-gelatin blend (PCLG) nanofibers prepared using electrospinning. Characterization by X-ray diffraction, X-ray photoelectron spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy, atomic force microscopy, and contact angle goniometry confirmed the presence of nCe on PCL or PCLG nanofibers (PCLG-Ce) of ≈300 nm fiber diameter. nCe-based PCLG scaffolds were cytocompatible with a variety of cell types, including primary cells. Primary cardiomyocytes cultured on nCe-decorated PCLG nanofibers showed marked reduction in the ROS levels when subjected to HO induced oxidative stress. Interestingly, we found that nCe-decorated PCLG nanofibers can suppress agonist-induced cardiac hypertrophy. Overall, the results of this study suggest the potential of nCe-decorated PCLG nanofibers as a cardiac patch with antioxidant and anti-hypertrophic properties.
活性氧（ROS）在心肌梗死（MI）后的再灌注缺血心脏组织中产生。心脏在MI后增强其功能表现的一种代偿性尝试是经历心肌细胞肥大。过去，降低心肌细胞中的ROS水平与抑制心脏肥大有关。值得注意的是，氧化铈纳米颗粒（nCe）已被广泛用于通过有效清除细胞ROS来保护细胞免受氧化损伤。此外，诸如纳米纤维之类的纤维基质正在成为用于制造可植入心脏贴片的有前景的基材。在本研究中，我们描述了使用静电纺丝制备的nCe修饰的聚己内酯（PCL）和PCL - 明胶共混物（PCLG）纳米纤维的制造。通过X射线衍射、X射线光电子能谱、能量色散X射线光谱、扫描电子显微镜、原子力显微镜和接触角测角法进行的表征证实了nCe存在于直径约300nm的PCL或PCLG纳米纤维（PCLG - Ce）上。基于nCe的PCLG支架与包括原代细胞在内的多种细胞类型具有细胞相容性。在nCe修饰的PCLG纳米纤维上培养的原代心肌细胞在受到HO诱导的氧化应激时，ROS水平显著降低。有趣的是，我们发现nCe修饰的PCLG纳米纤维可以抑制激动剂诱导的心脏肥大。总体而言，本研究结果表明nCe修饰的PCLG纳米纤维作为具有抗氧化和抗肥大特性的心脏贴片的潜力。
