Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
Nanobiotechnology Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
Biochim Biophys Acta Gen Subj. 2017 Feb;1861(2):386-395. doi: 10.1016/j.bbagen.2016.11.022. Epub 2016 Nov 15.
The design of efficient nerve conduits able to sustain the axonal outgrowth and its guidance towards appropriate targets is of paramount importance in nerve tissue engineering.
In this work, we propose the preparation of highly aligned nanocomposite fibers of gelatin/cerium oxide nanoparticles (nanoceria), prepared by electrospinning. Nanoceria are powerful self-regenerative antioxidant nanomaterials, that behave as strong reactive oxygen species scavengers, and among various beneficial effects, they have been proven to inhibit the cell senescence and to promote the neurite sprouting.
After a detailed characterization of the developed substrates, they have been tested on neuron-like SH-SY5Y cells, demonstrating strong antioxidant properties and beneficial multi-cue effects in terms of neurite development and alignment.
Obtained findings suggest efficiency of the proposed substrates in providing combined topographical stimuli and antioxidant effects to cultured cells.
Proposed nanocomposite scaffolds represent a promising approach for nerve tissue engineering and regenerative medicine.
设计能够维持轴突生长并引导其向适当靶标的高效神经导管在神经组织工程中至关重要。
在这项工作中，我们提出了通过静电纺丝制备明胶/氧化铈纳米粒子（纳米铈）的高度取向纳米复合纤维。纳米铈是一种强大的自再生抗氧化纳米材料，具有很强的活性氧物质清除能力，除了各种有益的作用外，还被证明可以抑制细胞衰老并促进神经突发芽。
在对所开发的基底材料进行详细表征后，将其在神经元样 SH-SY5Y 细胞上进行了测试，结果表明它们具有很强的抗氧化性能，并在神经突发育和定向方面具有有益的多线索效应。
研究结果表明，所提出的基底材料在为培养细胞提供联合的拓扑刺激和抗氧化作用方面具有高效性。
所提出的纳米复合支架代表了神经组织工程和再生医学的一种有前途的方法。
