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基因组展示了链接读取测序在鉴定推定基因方面的潜力。

Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative Gene.

机构信息

Laboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The Netherlands.

Population and Evolutionary Ecology Group, Institute of Ecology, FB02, University of Bremen, 28359 Bremen, Germany.

出版信息

Genes (Basel). 2020 Nov 24;11(12):1390. doi: 10.3390/genes11121390.

Abstract

is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, . It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of , complete with an ab initio-derived annotation and protein comparisons with fellow braconids, and . We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the gene ().

摘要

是一类广泛的鳞翅目幼虫外寄生蜂,包括几种重要作物的害虫,如玉米螟。它也是膜翅目动物中最早有记录的互补性别决定的案例之一。在这里,我们呈现了基于链接读取的基因组,包括从头预测的注释和与其他 Braconidae 物种、和的蛋白质比较。我们展示了链接读取组装在探索杂合区域方面的潜力,并寻找结构和同源性衍生的证据支持基因()。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/c763ed7c6530/genes-11-01390-g001.jpg

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