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基因组展示了链接读取测序在鉴定推定基因方面的潜力。

Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative Gene.

机构信息

Laboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The Netherlands.

Population and Evolutionary Ecology Group, Institute of Ecology, FB02, University of Bremen, 28359 Bremen, Germany.

出版信息

Genes (Basel). 2020 Nov 24;11(12):1390. doi: 10.3390/genes11121390.

DOI:10.3390/genes11121390
PMID:33255162
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7759789/
Abstract

is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, . It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of , complete with an ab initio-derived annotation and protein comparisons with fellow braconids, and . We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the gene ().

摘要

是一类广泛的鳞翅目幼虫外寄生蜂,包括几种重要作物的害虫,如玉米螟。它也是膜翅目动物中最早有记录的互补性别决定的案例之一。在这里,我们呈现了基于链接读取的基因组,包括从头预测的注释和与其他 Braconidae 物种、和的蛋白质比较。我们展示了链接读取组装在探索杂合区域方面的潜力,并寻找结构和同源性衍生的证据支持基因()。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/1432e1dfbe48/genes-11-01390-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/c763ed7c6530/genes-11-01390-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/5262b6c49908/genes-11-01390-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/027cff38e80e/genes-11-01390-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/1432e1dfbe48/genes-11-01390-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/c763ed7c6530/genes-11-01390-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/5262b6c49908/genes-11-01390-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/027cff38e80e/genes-11-01390-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b0d/7759789/1432e1dfbe48/genes-11-01390-g004.jpg

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本文引用的文献

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Haplotype tagging reveals parallel formation of hybrid races in two butterfly species.单体型标记揭示了两种蝴蝶物种中杂种种族的平行形成。
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Genome of the Parasitoid Wasp Diachasma alloeum, an Emerging Model for Ecological Speciation and Transitions to Asexual Reproduction.
寄生蜂 Diachasma alloeum 的基因组,生态物种形成和向无性繁殖过渡的新兴模式。
Genome Biol Evol. 2019 Oct 1;11(10):2767-2773. doi: 10.1093/gbe/evz205.
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Evidence for involvement of a transformer paralogue in sex determination of the wasp Leptopilina clavipes.一个转化同源基因参与黄蜂细腹潜蝇性别决定的证据。
Insect Mol Biol. 2018 Dec;27(6):780-795. doi: 10.1111/imb.12522. Epub 2018 Oct 1.
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Bioinformatics. 2018 Sep 15;34(18):3094-3100. doi: 10.1093/bioinformatics/bty191.
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Whole Genome Sequencing of the Braconid Parasitoid Wasp , an Important Biocontrol Agent of Pest Tepritid Fruit Flies.小茧蜂的全基因组测序,一种重要的害虫实蝇生物防治剂。
G3 (Bethesda). 2017 Aug 7;7(8):2407-2411. doi: 10.1534/g3.117.040741.
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