Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan.
Nihon Pharmaceutical Co., Ltd., Tokyo 1030012, Japan.
Mar Drugs. 2020 Nov 24;18(12):588. doi: 10.3390/md18120588.
Halocynthiaxanthin is an acetylenic carotenoid mainly found in . To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate . Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all- form and a small amount of forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with isomers being more abundant than the all- isomer in the mouse plasma and liver. In the small intestine, however, the all- isomer was primarily detected. The possibility that isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all--halocynthiaxanthin might be isomerized to the isomer after intestinal absorption.
海兔酮是一种炔属类胡萝卜素，主要存在于海鞘中。迄今为止，已有多项关于海兔酮的生物活性的研究报道，但它在哺乳动物体内的消化吸收机制尚未得到研究。本研究旨在评估海兔酮在小鼠体内的肠道吸收情况。我们从海鞘中提取了富含海兔酮的部位，以 5mg/kg 体重的剂量对小鼠进行经口给药。在摄入后 1、3、6 和 9 小时，使用 HPLC-PDA 定量分析了血浆、肝脏和小肠中的海兔酮含量。富含海兔酮的部位主要由全-形式和少量 15Z-形式组成。这三种异构体在摄入后 3 小时即可在小鼠血浆中检测到。在摄入该部位后发现了时间变化过程，在小鼠血浆和肝脏中，15Z-异构体比全-异构体更为丰富。然而，在小肠中，主要检测到全-异构体。不能排除 15Z-异构体可能从小肠迅速吸收的可能性，但我们的结果表明，膳食全--海兔酮可能在肠道吸收后异构化为 15Z-异构体。
