Department of Fermentation Technology and Microbiology, University of Agriculture in Krakow, 30-149 Krakow, Poland.
Biomolecules. 2020 Nov 25;10(12):1599. doi: 10.3390/biom10121599.
The bioavailability of minerals, such as zinc and magnesium, has a significant impact on the fermentation process. These metal ions are known to influence the growth and metabolic activity of yeast, but there are few reports on their effects on lactic acid bacteria (LAB) metabolism during sour brewing. This study aimed to evaluate the influence of magnesium and zinc ions on the metabolism of during the fermentation of brewers' wort. We carried out lactic acid fermentations using wort with different mineral compositions: without supplementation; supplemented with magnesium at 60 mg/L and 120 mg/L; and supplemented with zinc at 0.4 mg/L and 2 mg/L. The concentration of organic acids, pH of the wort and carbohydrate use was determined during fermentation, while aroma compounds, real extract and ethanol were measured after the mixed fermentation. The addition of magnesium ions resulted in the pH of the fermenting wort decreasing more quickly, an increase in the level of L-lactic acid (after 48 h of fermentation) and increased concentrations of some volatile compounds. While zinc supplementation had a negative impact on the strain, resulting in a decrease in the L-lactic acid content and a higher pH in the beer. We conclude that zinc supplementation is not recommended in sour beer production using .
矿物质(如锌和镁)的生物利用度对发酵过程有重大影响。这些金属离子已知会影响酵母的生长和代谢活性,但关于它们在酸酿过程中对乳酸菌(LAB)代谢的影响的报道很少。本研究旨在评估镁和锌离子对啤酒花发酵过程中代谢的影响。我们使用不同矿物质组成的麦芽汁进行乳酸发酵:不添加补充物;添加 60mg/L 和 120mg/L 的镁;以及添加 0.4mg/L 和 2mg/L 的锌。在发酵过程中测定有机酸的浓度、麦芽汁的 pH 值和碳水化合物的利用情况,而在混合发酵后测定香气化合物、实际提取物和乙醇的含量。添加镁离子会导致发酵麦汁的 pH 值更快下降,L-乳酸(发酵 48 小时后)水平升高,一些挥发性化合物的浓度增加。而锌的添加对菌株有负面影响,导致 L-乳酸含量降低,啤酒的 pH 值升高。我们得出结论,在使用生产酸啤酒时,不建议添加锌。