Borunova Seid-Fatima, Tkachev Nikolay, Iolchiev Baylar, Artyushina Zinaida, Abramov Pavel, Nikitina Marina, Silanteva Anastasia, Khusnetdinova Neilia, Serezhenkov Vladimir
The Russian State Center for Animal Feed and Drug Standardization and Quality (VGNKI), Zvenigorodskoye Shosse 5, 123022 Moscow, Russia.
Semenov Institute of Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, Building 1, 119991 Moscow, Russia.
Vet Sci. 2020 Nov 29;7(4):191. doi: 10.3390/vetsci7040191.
Diseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the development of inflammation and oxidative stress, can be associated with a lack of the nitrogen monoxide which controls many signaling pathways in the body. The level of the nitric oxide (NO) is involved in the regulation of the immune and nervous systems, the tone of all the blood vessels, and the courses of many pathological processes. The nitric oxide activates guanylate cyclase (sGC) and leads to vascular relaxation. The aim of this investigation was to study the metabolites of nitric oxide in horses suffered from intestinal diseases. The levels of nitric oxide in the blood serum of horses depending on their age and health state was studied. The concentration of nitrites in the blood serum of horses aged 6-25 years was 3.4 ± 4.2 μM, and in the young horses (1-5 years) the level of this indicator was 8.2 ± 5.4 μM. A sharp decrease in nitrite was observed in all the horses with intestinal diseases of 2 ± 0.9 μM, especially with tympanitic caecun of 0.6 ± 0.4 μM and with spasmodic colic of 1.8 ± 0.5 μM. The level of nitrosylhemoglobin HbNO in the blood of the diseased animals was higher than that in clinically healthy horses, regardless of age.
马胃肠道疾病由多种因素引起，发病机制复杂。开发有效的鉴别诊断方法具有高度的基础和应用价值。伴有炎症和氧化应激发展的马消化道疾病的发病机制，可能与体内控制多种信号通路的一氧化氮缺乏有关。一氧化氮（NO）水平参与免疫和神经系统调节、所有血管的张力以及许多病理过程的进程。一氧化氮激活鸟苷酸环化酶（sGC）并导致血管舒张。本研究的目的是研究患肠道疾病马匹体内一氧化氮的代谢产物。研究了不同年龄和健康状况的马血清中一氧化氮的水平。6至25岁马的血清中亚硝酸盐浓度为3.4±4.2μM，而幼马（1至5岁）该指标水平为8.2±5.4μM。所有患肠道疾病的马亚硝酸盐水平均急剧下降至2±0.9μM，尤其是患气胀性盲肠的马为0.6±0.4μM，患痉挛性绞痛的马为1.8±0.5μM。患病动物血液中的亚硝基血红蛋白HbNO水平高于临床健康的马，与年龄无关。
