一种用于免疫组织化学分析的将细胞共价连接到载玻片上的方法。
A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays.
作者信息
Maples J A
出版信息
Am J Clin Pathol. 1985 Mar;83(3):356-63. doi: 10.1093/ajcp/83.3.356.
PMID:2579542
Abstract
A method for covalently attaching viable cells to glass slides is described. The cells are linked covalently to the slide without the use of drying or other fixation procedures that normally destroy cellular surface antigens. The attachment is permanent, and the slides with attached cells may be cryopreserved. The use of the avidin-biotin-peroxidase procedure with this procedure shows excellent sensitivity comparable to flow cytometry.
摘要
描述了一种将活细胞共价连接到载玻片上的方法。细胞可共价连接到载玻片上，而无需使用通常会破坏细胞表面抗原的干燥或其他固定程序。这种连接是永久性的，带有附着细胞的载玻片可以进行冷冻保存。将抗生物素蛋白-生物素-过氧化物酶程序与该程序一起使用时，显示出与流式细胞术相当的出色灵敏度。
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