Salvin S B, Sonnenfeld G, Nishio J
Infect Immun. 1977 Sep;17(3):639-43. doi: 10.1128/iai.17.3.639-643.1977.
Mice sensitized intravenously with 300 microgram of BCG cell walls in Drakeol-Tween 80 and challenged intravenously 3 weeks later with 50 mg of old tuberculin released migration inhibitory factor (MIF) into the circulating blood in quantities that could be detected in serum dilutions of 1:64 to 1:128. When thymus-derived lymphocytes (T-cells) were absent at the time of sensitization, as in neonatally thymectomized mice or in athymic nude mice, detectable amounts of MIF were not formed. Sensitized mice treated with either anti-theta serum or anti-bone marrow-derived lymphocyte (B-cell) serum before intravenous challenge with old tuberculin released reduced amounts of MIF into the circulation. Mice lethally irradiated, reconstituted with B-cells, sensitized with BCG cell walls, and then challenged intravenously 3 weeks later with old tuberculin did not release MIF into the circulation. When T-cells were injected at least 10 days before challenge, however, MIF appeared.
用300微克卡介苗细胞壁在石蜡油 - 吐温80中静脉致敏的小鼠，3周后静脉注射50毫克旧结核菌素，会释放出可在1:64至1:128血清稀释度中检测到的迁移抑制因子（MIF）进入循环血液。当致敏时不存在胸腺来源的淋巴细胞（T细胞），如新生期胸腺切除的小鼠或无胸腺裸鼠，就不会形成可检测量的MIF。在用旧结核菌素静脉攻击前，用抗θ血清或抗骨髓来源淋巴细胞（B细胞）血清处理的致敏小鼠，向循环中释放的MIF量减少。经致死剂量照射、用B细胞重建、用卡介苗细胞壁致敏、3周后静脉注射旧结核菌素攻击的小鼠，不会向循环中释放MIF。然而，当在攻击前至少10天注射T细胞时，MIF会出现。
