Tomino Y, Sakai H, Woodroffe A J, Clarkson A R
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
Acta Pathol Jpn. 1987 Nov;37(11):1763-7. doi: 10.1111/j.1440-1827.1987.tb02869.x.
A study of the solubilization of glomerular immune deposits by serum or complement in patients with IgA nephropathy is described. Renal biopsy specimens were obtained from 15 patients with IgA nephropathy. These specimens were incubated with fresh and heated sera from healthy adults or with lyophilized complement components, i.e., C3 and C4, at 37 degrees C for one hour in plastic tubes. The sections were then stained with fluorescein isothiocyanate (FITC)-labelled anti-human IgA antisera and examined by fluorescence microscopy. Normal sera showed a marked capacity to solubilize the glomerular immune deposits characteristic of IgA nephropathy. The solubilization capacity was reduced after inactivation and absorption of sera with anti-human C3 antiserum. Lyophilized C3 or C4 did not show any ability to solubilize such deposits. It was concluded that the solubilization of glomerular immune deposits may require whole active (fresh) components of complement related to the alternative pathway.
本文描述了一项关于IgA肾病患者血清或补体对肾小球免疫沉积物溶解作用的研究。从15例IgA肾病患者获取肾活检标本。将这些标本与健康成年人的新鲜血清和加热血清，或与冻干补体成分（即C3和C4）在塑料管中于37℃孵育1小时。然后用异硫氰酸荧光素（FITC）标记的抗人IgA抗血清对切片进行染色，并通过荧光显微镜检查。正常血清显示出显著的溶解IgA肾病特征性肾小球免疫沉积物的能力。在用抗人C3抗血清使血清失活和吸收后，溶解能力降低。冻干的C3或C4未显示出溶解此类沉积物的任何能力。得出的结论是，肾小球免疫沉积物的溶解可能需要与替代途径相关的完整活性（新鲜）补体成分。
