Eguchi K, Tomino Y, Yagame M, Miyazaki M, Takiura F, Miura M, Suga T, Endoh M, Nomoto Y, Sakai H
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
Tokai J Exp Clin Med. 1987 Dec;12(5-6):331-5.
Double immunofluorescent studies on IgA, poly (MAC) or C3 in glomeruli from patients with IgA nephropathy are described. Renal biopsy specimens were obtained from 12 patients with IgA nephropathy, four patients with proliferative glomerulonephritis (PGN) and two normal human kidney (NHK). These specimens were incubated with monoclonal anti-poly C9 (membrane attack complex; MAC) and then stained with FITC-labelled goat anti-mouse immunoglobulin (Ig) antiserum. After washing with phosphate buffered saline (PBS) (pH 7.4), the sections were stained with rhodamine-labelled rabbit anti-human IgA antiserum and examined by fluorescence microscopy. The sections were also stained with FITC-labelled goat anti-human C3 antiserum and then stained with rhodamine-labelled rabbit anti-human IgA antiserum. Markedly combined depositions of IgA and poly C9 or C3 in glomeruli were observed in patients with IgA nephropathy. There was a significant correlation between the deposition of poly C9 and the grading of histopathological injuries in such patients. There was also a significant correlation between the deposition of poly C9 in the extraglomerular vascular vessels and the ageing in patients with IgA nephropathy, PGN and NHK. It appears that the deposition of poly C9 might detect directly the activities of complement in glomeruli from patients with IgA nephropathy.
描述了对IgA肾病患者肾小球中的IgA、多聚（膜攻击复合物；MAC）或C3进行的双重免疫荧光研究。从12例IgA肾病患者、4例增生性肾小球肾炎（PGN）患者和2例正常人类肾脏（NHK）获取肾活检标本。将这些标本与单克隆抗多聚C9（膜攻击复合物；MAC）孵育，然后用异硫氰酸荧光素（FITC）标记的山羊抗小鼠免疫球蛋白（Ig）抗血清染色。用磷酸盐缓冲盐水（PBS）（pH 7.4）洗涤后，切片用罗丹明标记的兔抗人IgA抗血清染色，并通过荧光显微镜检查。切片还用FITC标记的山羊抗人C3抗血清染色，然后用罗丹明标记的兔抗人IgA抗血清染色。在IgA肾病患者的肾小球中观察到IgA与多聚C9或C3的明显联合沉积。在这类患者中，多聚C9的沉积与组织病理学损伤分级之间存在显著相关性。在IgA肾病、PGN和NHK患者中，肾小球外血管中多聚C9的沉积与年龄之间也存在显著相关性。看来多聚C9的沉积可能直接检测到IgA肾病患者肾小球中补体的活性。
