用于检测沙眼衣原体抗原的酶免疫测定法和酶联荧光免疫测定法。

Enzyme immunoassay and enzyme-linked fluoroimmunoassay for detection of Chlamydia trachomatis antigen.

作者信息

Leinonen M, Saikku P, Nurminen M, Lassus A

机构信息

National Public Health Institute, Helsinki, Finland.

出版信息

Eur J Clin Microbiol. 1987 Dec;6(6):659-61. doi: 10.1007/BF02013064.

DOI:10.1007/BF02013064

PMID:3326744

Abstract

An enzyme immunoassay (EIA) and an enzyme-linked fluoroimmunoassay (ELFIA) utilizing monoclonal antibody to major outer membrane protein of Chlamydia trachomatis L2 were developed for rapid detection of chlamydial antigen in clinical samples. The EIA and ELFIA could detect levels of purified chlamydial outer membrane protein as low as 1.0 and 0.2 ng/ml respectively. However, when EIA and ELFIA were compared to chlamydial isolation using 160 patient samples, the sensitivity rate was 68% and 85% respectively. The sensitivity of the antigen detection method might be increased by simply using less diluted samples than in the present study. Chlamydial antigen was also demonstrated by EIA and ELFIA in 15% and 23% of culture-negative samples. The reason for these false-positive findings remains undetermined.

摘要

利用针对沙眼衣原体L2主要外膜蛋白的单克隆抗体，开发了一种酶免疫测定法（EIA）和一种酶联荧光免疫测定法（ELFIA），用于快速检测临床样本中的衣原体抗原。EIA和ELFIA分别能检测低至1.0和0.2 ng/ml的纯化衣原体外膜蛋白水平。然而，当使用160份患者样本将EIA和ELFIA与衣原体分离法进行比较时，灵敏度分别为68%和85%。通过简单地使用比本研究中稀释度更低的样本，抗原检测方法的灵敏度可能会提高。在15%和23%的培养阴性样本中，EIA和ELFIA也检测出了衣原体抗原。这些假阳性结果的原因仍未确定。