Minnikin D E, Dobson G, Parlett J H, Goodfellow M, Magnusson M
Department of Organic Chemistry, University, Newcastle upon Tyne, UK.
Eur J Clin Microbiol. 1987 Dec;6(6):703-7. doi: 10.1007/BF02013082.
Extracts of representative mycobacteria were examined by thin-layer chromatography for glycosylphenolphthiocerol dimycocerosates. The glycolipid typical of Mycobacterium bovis was also found in Mycobacterium africanum and Mycobacterium microti, but it was absent in Mycobacterium bovis AN 5. Mycobacterium gastri strains contained a glycolipid which was chromatographically similar to that in Mycobacterium kansasii. Representatives of Mycobacterium marinum produced a distinct glycolipid type, and one strain had major amounts of a more polar variant. The sugar moieties of purified lipids, including that from Mycobacterium leprae, were identified by thin-layer chromatography of methyl glycosides in acid methanolysates.
采用薄层色谱法对代表性分枝杆菌提取物进行糖基酚硫脂二霉菌酸酯检测。牛分枝杆菌特有的糖脂在非洲分枝杆菌和微小分枝杆菌中也有发现，但在牛分枝杆菌AN 5中未发现。胃分枝杆菌菌株含有一种糖脂，其色谱特征与堪萨斯分枝杆菌中的相似。海分枝杆菌的代表菌株产生一种独特的糖脂类型，其中一个菌株含有大量极性更强的变体。通过对酸性甲醇解产物中甲基糖苷的薄层色谱分析，鉴定了包括麻风分枝杆菌在内的纯化脂质的糖部分。
