sp. nov., a novel addition to the genus, isolated from the soil of a cultivated maize field.

A Gram-negative, rod-shaped bacterium, strain DN04, was isolated from the soil of a maize field in North Carolina, USA. Based on the 16S rRNA gene sequence, the most similar species are Sac-22, DCY83, and Sac-41 with a 97.8, 97.6, or 96.9 % sequence similarity, respectively. We compared the biochemical phenotype of DN04 to Sac-22 and 115 and other reference strains from different genera within the Oxalobacteraceae and while the biochemical profile of DN04 is most similar to Sac-22 and other and strains, there are also distinct differences. DN04 can for example utilize turanose, N-acetyl-d-glucosamine, inosine, and l-pyroglutamic acid. The four fatty acids found in the highest percentages were C iso (24.6 %), C isoG (19.4 %), C iso3-OH (16.8 %), and summed feature 3 (C ⍵7c and/or C ⍵6c) (12.5 %). We also applied whole genome sequencing to determine if DN04 is a novel species. The most similar AAI (average amino acid identity) score was 70.8 % ( NZ CP038026), and the most similar ANI (average nucleotide identity) score was 84.8 % ( KCTC 22382), which indicates that DN04 is a novel species. The genome-to-genome-distance calculation (GGDC) revealed a DDH of 28.3 % to KCTC 22382, which is much lower than the new species threshold. Based on the morphological, phenotypic, and genomic differences, we propose sp. nov. as a novel species within the genus (type strain DN04=NRRL B-65552=LMG 31736).