School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Longgang District, Shenzhen, Guangdong Province 518172, China.
Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Longgang District, Shenzhen, Guangdong Province 518172, China.
Nucleic Acids Res. 2021 Jan 8;49(D1):D1268-D1275. doi: 10.1093/nar/gkaa1104.
DNA methylation is an important epigenetic regulator in gene expression and has several roles in cancer and disease progression. MethHC version 2.0 (MethHC 2.0) is an integrated and web-based resource focusing on the aberrant methylomes of human diseases, specifically cancer. This paper presents an updated implementation of MethHC 2.0 by incorporating additional DNA methylomes and transcriptomes from several public repositories, including 33 human cancers, over 50 118 microarray and RNA sequencing data from TCGA and GEO, and accumulating up to 3586 manually curated data from >7000 collected published literature with experimental evidence. MethHC 2.0 has also been equipped with enhanced data annotation functionality and a user-friendly web interface for data presentation, search, and visualization. Provided features include clinical-pathological data, mutation and copy number variation, multiplicity of information (gene regions, enhancer regions, and CGI regions), and circulating tumor DNA methylation profiles, available for research such as biomarker panel design, cancer comparison, diagnosis, prognosis, therapy study and identifying potential epigenetic biomarkers. MethHC 2.0 is now available at http://awi.cuhk.edu.cn/∼MethHC.
DNA 甲基化是基因表达中一种重要的表观遗传调控因子，在癌症和疾病进展中具有多种作用。MethHC 版本 2.0（MethHC 2.0）是一个集成的、基于网络的资源，专注于人类疾病（特别是癌症）的异常甲基组。本文介绍了通过整合来自多个公共存储库的额外 DNA 甲基组和转录组对 MethHC 2.0 的更新实现，包括 33 种人类癌症、来自 TCGA 和 GEO 的超过 50118 个微阵列和 RNA 测序数据，以及积累了来自超过 7000 份具有实验证据的已发表文献的 3586 条手动整理数据。MethHC 2.0 还配备了增强的数据注释功能和用户友好的网络界面，用于数据呈现、搜索和可视化。提供的功能包括临床病理数据、突变和拷贝数变异、多重信息（基因区域、增强子区域和 CGI 区域）以及循环肿瘤 DNA 甲基化谱，可用于研究标志物面板设计、癌症比较、诊断、预后、治疗研究和识别潜在的表观遗传生物标志物。MethHC 2.0 可在 http://awi.cuhk.edu.cn/∼MethHC 上获得。
