Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Sci Rep. 2020 Dec 3;10(1):21205. doi: 10.1038/s41598-020-77723-6.
Halobacteria, a class of Euryarchaeota are extremely halophilic archaea that can adapt to a wide range of salt concentration generally from 10% NaCl to saturated salt concentration of 32% NaCl. It consists of the orders: Halobacteriales, Haloferaciales and Natriabales. Pan-genome analysis of class Halobacteria was done to explore the core (300) and variable components (Softcore: 998, Cloud:36531, Shell:11784). The core component revealed genes of replication, transcription, translation and repair, whereas the variable component had a major portion of environmental information processing. The pan-gene matrix was mapped onto the core-gene tree to find the ancestral (44.8%) and derived genes (55.1%) of the Last Common Ancestor of Halobacteria. A High percentage of derived genes along with presence of transformation and conjugation genes indicate the occurrence of horizontal gene transfer during the evolution of Halobacteria. A Core and pan-gene tree were also constructed to infer a phylogeny which implicated on the new super-order comprising of Natrialbales and Halobacteriales.
嗜盐古菌是广盐古菌的一个类群，可适应从 10%氯化钠到 32%饱和盐浓度的盐度范围。它由三个目组成：盐杆菌目、盐球菌目和盐盒菌目。对嗜盐古菌类群进行了泛基因组分析，以探索核心（300）和可变组件（软核心：998、云：36531、壳：11784）。核心组件揭示了复制、转录、翻译和修复的基因，而可变组件则包含了大部分环境信息处理的基因。泛基因矩阵被映射到核心基因树上，以找到嗜盐古菌最后共同祖先的祖先（44.8%）和衍生基因（55.1%）。大量的衍生基因以及转化和转导基因的存在表明，在嗜盐古菌的进化过程中发生了水平基因转移。还构建了核心和泛基因树来推断系统发育，这暗示了新的超级目，包括盐盒菌目和盐杆菌目。
