Nag Papri, Mondal Nibendu, Sarkar Jagannath, Das Sampa
Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII M, Kolkata, West Bengal, 700 054, India.
Department of Microbiology, Bose Institute, P/12 C.I.T. Scheme VII M, Kolkata, West Bengal, 700 054, India.
Arch Microbiol. 2022 May 25;204(6):347. doi: 10.1007/s00203-022-02960-4.
Paraburkholderia bengalensis sp. nov. strain IR64_4_BI was isolated from rice roots cultivated in Madhyamgram field station of Bose Institute, West Bengal, India. IR64_4_BI is a Gram-negative, motile, nitrate-reducing, nitrogen-fixing bacterium. Whole-cell fatty acid analyses of IR64_4_BI show C16:0, summed feature 8 (comprising C18:1ω7c and/or C18:1 ω 6c) and summed feature 3(C16:1 w7c/C16:1 w6c or C16:1 ω 7c/C16:1 ω 6c) were the predominant fatty acids. 16S rRNA phylogeny showed that it was most similar to P. phymatum STM815 (98.5% identity), P. terrae KMY02 (98.44% identity) and P. hospita LMG 20598T (98.32% identity). The Average Nucleotide Identity-BLAST (ANIb) of P. bengalensis IR64_4_BI with P. hospita DSM 17164, P. terrae DSM 17804, P. phymatum STM815 and P. hospita LMG 20598T was 83.11, 83.52, 84.5 and 83.12% respectively. Comparison of genome sequence of IR64_4_BI with other species of Paraburkholderia using the Multi-locus species tree software show that P. bengalensis IR64_4_BI is a novel species. The ability of P. bengalensis IR64_4_BI to survive on nitrogen-free medium under microaerophilic conditions and the abundance of nitrogen metabolism-related genes makes this strain a potential candidate for developing a nitrogen-fixing system in rice. Based on genotypic, phenotypic and chemotaxonomic studies, we propose that IR64_4_BI (= MTCC 13051 = JCM 34777) is a new species of Paraburkholderia which has been assigned as Paraburkholderia bengalensis sp.nov.
孟加拉伯克霍尔德氏菌新种菌株IR64_4_BI是从印度西孟加拉邦博斯研究所马德亚姆格拉姆田间站种植的水稻根部分离得到的。IR64_4_BI是一种革兰氏阴性、可运动、能还原硝酸盐、固氮的细菌。对IR64_4_BI的全细胞脂肪酸分析表明，C16:0、总和特征8（包括C18:1ω7c和/或C18:1 ω 6c）以及总和特征3（C16:1 w7c/C16:1 w6c或C16:1 ω 7c/C16:1 ω 6c）是主要脂肪酸。16S rRNA系统发育分析表明，它与费氏伯克霍尔德氏菌STM815（同一性为98.5%）、土壤伯克霍尔德氏菌KMY02（同一性为98.44%）和栖居伯克霍尔德氏菌LMG 20598T（同一性为98.32%）最为相似。孟加拉伯克霍尔德氏菌IR64_4_BI与栖居伯克霍尔德氏菌DSM 17164、土壤伯克霍尔德氏菌DSM 17804、费氏伯克霍尔德氏菌STM815和栖居伯克霍尔德氏菌LMG 20598T的平均核苷酸同一性-比对（ANIb）分别为83.11%、83.52%、84.5%和83.12%。使用多位点物种树软件将IR64_4_BI的基因组序列与其他伯克霍尔德氏菌属物种进行比较，结果表明孟加拉伯克霍尔德氏菌IR64_4_BI是一个新物种。孟加拉伯克霍尔德氏菌IR64_4_BI在微需氧条件下在无氮培养基上存活的能力以及丰富的氮代谢相关基因，使得该菌株成为开发水稻固氮系统的潜在候选菌株。基于基因型、表型和化学分类学研究，我们提议IR64_4_BI（=MTCC 13051=JCM 34777）是伯克霍尔德氏菌属的一个新物种，已被命名为孟加拉伯克霍尔德氏菌新种。
