Gu Li, Su Ting, An Ming-Tai, Hu Guo-Xiong
College of Life Sciences, Guizhou University, Guiyang 550025, China.
The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region Ministry of Education, Guizhou University, Guiyang 550025, China.
Plants (Basel). 2020 Dec 2;9(12):1692. doi: 10.3390/plants9121692.
, a member of Gesneriaceae, is known as , which has been regarded a synonym of the former. The species is endemic to Guizhou, southwestern China, and is evaluated as vulnerable (VU) under the International Union for Conservation of Nature (IUCN) criteria. Until now, the sequence and genome information of remains unknown. In this study, we assembled and characterized the complete chloroplast (cp) genome of using Illumina sequencing data for the first time. The total length of the cp genome was 154,069 bp with a typical quadripartite structure consisting of a pair of inverted repeats (IRs) of 25,392 bp separated by a large single copy region (LSC) of 85,156 bp and a small single copy region (SSC) of18,129 bp. The genome comprised 114 unique genes with 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. Thirty-one repeat sequences and 74 simple sequence repeats (SSRs) were identified. Genome alignment across five plastid genomes of Gesneriaceae indicated a high sequence similarity. Four highly variable sites (, , , and ) were identified. Phylogenetic analysis indicated that grouped together with , supporting resurrection of the name from . The complete cp genome sequence will contribute to further studies in molecular identification, genetic diversity, and phylogeny.
苦苣苔科的一个成员，被称为[具体名称缺失]，它曾被视为前者的同义词。该物种为中国西南部贵州省特有，根据国际自然保护联盟（IUCN）标准被评估为易危（VU）。到目前为止，[具体物种名称缺失]的序列和基因组信息仍然未知。在本研究中，我们首次使用Illumina测序数据组装并表征了[具体物种名称缺失]的完整叶绿体（cp）基因组。cp基因组全长154,069 bp，具有典型的四分体结构，由一对25,392 bp的反向重复序列（IRs）组成，中间被一个85,156 bp的大单拷贝区域（LSC）和一个18,129 bp的小单拷贝区域（SSC）隔开。该基因组包含114个独特基因，其中有80个蛋白质编码基因、30个tRNA基因和4个rRNA基因。鉴定出31个重复序列和74个简单序列重复（SSRs）。对苦苣苔科五个质体基因组的基因组比对表明序列相似性很高。鉴定出四个高度可变位点（[具体位点缺失]、[具体位点缺失]、[具体位点缺失]和[具体位点缺失]）。系统发育分析表明[具体物种名称缺失]与[具体物种名称缺失]聚在一起，支持从[具体名称缺失]中恢复[具体名称缺失]这个名称。完整的cp基因组序列将有助于分子鉴定、遗传多样性和系统发育的进一步研究。
