Van Herreweghen Florence, De Paepe Kim, Marzorati Massimo, Van de Wiele Tom
Center for Microbial Ecology and Technology (CMET), Ghent University, Ghent, Belgium.
Prodigest, Ghent, Belgium.
Appl Environ Microbiol. 2021 Mar 1;87(4). doi: 10.1128/AEM.02647-20. Epub 2020 Dec 4.
Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put in the spotlight. As a mucin degrader, colonizes the interesting but not-fully described host-glycan degradation niche., . Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of administration on the endogenous community while also taking into account its nutritional specificity. As such, the effect of administration was investigated with and without addition of mucin. This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals. It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic. Preliminary and ongoing research is mostly based on mouse models and human intervention trials. While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of effects at the level of the microbiome. We aimed to evaluate the effect of treatment on the endogenous community from four different donors in a validated, controlled model of the gut microbial ecosystem (SHIME). Taking into account the nutritional specificity of , and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, and the endogenous community was investigated. The effects on the microbial community composition and functionality of supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota. Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including cluster and This study revealed that the supplementation of together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.
肠道健康生物标志物的识别以及调节微生物群组成和活性以改善健康的方法的研究已成为焦点。作为一种粘蛋白降解菌，定植于有趣但尚未完全描述的宿主聚糖降解生态位。关于嗜粘蛋白阿克曼菌已经开展了大量研究，但对于其在结肠复杂微生物生态系统中的行为、粘蛋白影响嗜粘蛋白阿克曼菌行为的潜在作用以及其益生菌给药对微生物生态系统的影响知之甚少。本研究旨在调查给药对内源群落的影响，同时考虑其营养特异性。因此，在添加和不添加粘蛋白的情况下研究了给药的效果。这使我们能够阐明粘蛋白的存在对于调节益生菌补充效率的重要性。嗜粘蛋白阿克曼菌是一种大量存在的共生粘蛋白降解肠道细菌（1%-4%），广泛分布于健康个体中。它已被定位为一种健康生物标志物，目前正在作为一种生物治疗剂和下一代益生菌进行探索。初步和正在进行的研究大多基于小鼠模型和人体干预试验。虽然这些研究能够评估生理相关终点，但粪便样本分析在微生物组水平上对嗜粘蛋白阿克曼菌作用的深入机制表征方面存在局限性。我们旨在通过经过验证的、可控的肠道微生物生态系统模拟模型（SHIME）评估嗜粘蛋白阿克曼菌处理对来自四个不同供体的内源群落的影响。考虑到嗜粘蛋白阿克曼菌的营养特异性以及粘蛋白在结肠环境中的益生元样作用，研究了粘蛋白、嗜粘蛋白阿克曼菌和内源群落之间的相互作用。不添加粘蛋白时，嗜粘蛋白阿克曼菌补充对微生物群落组成和功能的影响有限，而添加粘蛋白成功诱导了肠道微生物群的组成和代谢变化。事实上，添加粘蛋白导致所有四个供体的乙酸盐、丙酸盐和丁酸盐产量显著更高，以及包括阿克曼菌属簇和双歧杆菌属在内的几种菌种增加。本研究表明，嗜粘蛋白阿克曼菌与粘蛋白一起补充会限制粘蛋白在诱导组成变化方面观察到的益生元样作用。
