Federal University of the South and Southeast of Pará (Unifesspa), Xinguara, Brazil.
Federal Rural University of the Semi-Arid Zone, Mossoró, Brazil.
Vet Clin Pathol. 2020 Dec;49(4):545-556. doi: 10.1111/vcp.12917. Epub 2020 Dec 5.
Hemotherapy in ruminants is limited to whole blood transfusions, sometimes with stored blood for up to 42 days, but little attention has been given to the effect of blood storage times and recipient responses after transfusions.
We aimed to evaluate the hematologic and serum biochemical effects after allogeneic blood transfusion with either fresh or stored blood in sheep. We also sought to examine hematologic and biochemical analyte changes in the store blood.
Eighteen sheep underwent a single phlebotomy to remove 40% of their blood volume. The sheep were divided into three experimental groups, G0, G15, and G35, which included six animals, each receiving 20 mL/kg of either fresh blood or blood stored in citrate, phosphate, dextrose, and adenine (CPDA-1) bags for 15 and 35 days, respectively. Biochemical, hematologic, coagulation, blood gas, lipid peroxidation, and oxidative stress test evaluations were performed using the blood samples gathered at T0 (before transfusion), 30 minutes (T30m), 6, 12, 24, 48, 72, and 96 hours (T6h-T96h), 8 days (T8d), and 16 days (T16d) after transfusions.
Sheep exhibited increases in packed cell volumes, red blood cell counts, and total hemoglobin concentrations at T30m (P < .05). G35 animals had greater plasma hemoglobin concentrations at T12h and decreased blood pH values at T6h, characterized by slight metabolic acidemia. Regarding oxidative stress, G35 animals had decreased catalase activities from T0 at T30m, T6h, T12h, and T24h, indicating that hemolysis had occurred, which was supported by concomitant increases in bilirubin.
Sheep transfused with 35-day stored blood exhibited greater hematologic, blood gas, biochemical, and oxidative alterations; however, anemic animals without comorbidities effectively reversed those alterations.
反刍动物的血液疗法仅限于全血输注，有时会使用储存血，储存时间最长可达 42 天，但很少关注血液储存时间和输注后受体反应的影响。
我们旨在评估绵羊同种异体输血后新鲜血或储存血的血液学和血清生化效应。我们还试图检查储存血中的血液学和生化分析物变化。
18 只绵羊进行单次采血以去除其 40%的血容量。绵羊分为三个实验组，G0、G15 和 G35，每组 6 只动物，分别接受 20mL/kg 的新鲜血液或储存在柠檬酸盐、磷酸盐、葡萄糖和腺嘌呤（CPDA-1）袋中的血液，储存时间分别为 15 天和 35 天。使用 T0（输血前）、30 分钟（T30m）、6、12、24、48、72 和 96 小时（T6h-T96h）、8 天（T8d）和 16 天（T16d）采集的血液样本进行生化、血液学、凝血、血气、脂质过氧化和氧化应激试验评估。
绵羊在 T30m 时表现出红细胞压积、红细胞计数和总血红蛋白浓度的增加（P<.05）。G35 动物在 T12h 时具有更高的血浆血红蛋白浓度，在 T6h 时具有更低的血液 pH 值，表现为轻度代谢性酸中毒。关于氧化应激，G35 动物从 T0 在 T30m、T6h、T12h 和 T24h 时的过氧化氢酶活性降低，表明发生了溶血，这与胆红素的同时增加相吻合。
输注 35 天储存血的绵羊表现出更大的血液学、血气、生化和氧化变化；然而，没有合并症的贫血动物有效地逆转了这些变化。
