Matsuzaki Kazuma, Tatsumi Daisuke, Sugiyama Takeharu, Hiraoka Masanori, Igura Noriyuki, Tsubaki Shuntaro
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan.
Faculty of Agriculture, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan.
ACS Omega. 2024 Jun 25;9(27):29896-29903. doi: 10.1021/acsomega.4c04080. eCollection 2024 Jul 9.
sp. is a type of green algae and is widely distributed in coastal areas around the world due to eutrophication. Effective utilization of sp. is expected for the establishment of carbon-neutral biochemical production. Microwave-assisted hydrothermal extraction is one of the most efficient ways of extracting highly functional polysaccharides called ulvan. Here, we demonstrate the importance of microwave frequency in enhancing ulvan extraction from . We found that microwaves (2.45 GHz) selectively heat water solvent, while radio frequency (200 MHz) selectively heats ionic ulvan. Moreover, 2.45 GHz was more effective for extracting ulvan than 200 MHz. Then, we analyzed the conformational change in ulvan during microwave irradiation using in situ small-angle X-ray scattering. Microwaves initiated the loosening of ulvan bundles at temperatures lower than those of conventional heating. As a result, microwaves at 2.45 GHz selectively heat water and initiate ulvan structural change to enhance the extraction of ulvan from .
某藻是一种绿藻，由于富营养化而广泛分布于世界各地的沿海地区。预计有效利用某藻对于建立碳中和生化生产具有重要意义。微波辅助水热萃取是提取名为岩藻聚糖硫酸酯的高功能多糖的最有效方法之一。在此，我们证明了微波频率在增强从某藻中提取岩藻聚糖硫酸酯方面的重要性。我们发现，微波（2.45吉赫兹）选择性地加热水溶剂，而射频（200兆赫兹）选择性地加热离子化的岩藻聚糖硫酸酯。此外，2.45吉赫兹在提取岩藻聚糖硫酸酯方面比200兆赫兹更有效。然后，我们使用原位小角X射线散射分析了微波辐照过程中岩藻聚糖硫酸酯的构象变化。微波在低于传统加热的温度下引发岩藻聚糖硫酸酯束的松弛。结果，2.45吉赫兹的微波选择性地加热水并引发岩藻聚糖硫酸酯结构变化，以增强从某藻中提取岩藻聚糖硫酸酯。
