Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, United States; Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, United States; Ministry of Agriculture and Rural Affairs Feed Industry Centre, China Agricultural University, Beijing, 100193, PR China.
Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, United States; Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, United States.
Carbohydr Polym. 2021 Feb 1;253:117309. doi: 10.1016/j.carbpol.2020.117309. Epub 2020 Oct 27.
Bacterial resistance to antibiotics is a critical public health concern. Alternatives of antibiotics are needed urgently. Herein, we designed and engineered a new nano-antimicrobial, chitosan nanoparticles (CNs)-antimicrobial peptide microcin J25 (MccJ25) conjugates (CNMs). The engineered CNMs proved to be highly active against Gram-negative and Gram-positive bacteria, and the activity of CNMs and CNs was stable in various thermal and pH environments. Escherichia coli K88 strain treated with CNMs did not acquire resistance in serial passage assays over a period of 18 days. Risk assessment with cell lines showed that CNMs did not cause toxicity. Additionally, CNMs did not reduce the lifespan of C. elegans. In summary, this study demonstrated that CNMs can serve as an excellent novel antimicrobial agent against multi-drug resistance pathogens.
细菌对抗生素的耐药性是一个严重的公共卫生问题。迫切需要抗生素的替代品。在此，我们设计并构建了一种新型纳米抗菌剂，壳聚糖纳米颗粒（CNs）-抗菌肽微菌素 J25（MccJ25）缀合物（CNMs）。工程化的 CNMs 被证明对革兰氏阴性和革兰氏阳性菌具有高度活性，并且 CNMs 和 CNs 的活性在各种热和 pH 环境中均稳定。在 18 天的连续传代实验中，用 CNMs 处理的大肠杆菌 K88 菌株没有获得耐药性。用细胞系进行的风险评估表明，CNMs 不会引起毒性。此外，CNMs 不会缩短秀丽隐杆线虫的寿命。总之，这项研究表明，CNMs 可以作为一种对抗多药耐药病原体的优秀新型抗菌剂。
