German Institute of Human Nutrition, Potsdam - Rehbrücke, Department of Nutrition and Gerontology, Nuthetal, Germany.
German Institute of Human Nutrition, Potsdam - Rehbrücke, Department of Nutrition and Gerontology, Nuthetal, Germany; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Research Group on Geriatrics, Germany; German Center for Diabetes Research (DZD), München-Neuherberg, Germany.
Exp Gerontol. 2021 Feb;144:111177. doi: 10.1016/j.exger.2020.111177. Epub 2020 Dec 3.
Fatigue is a complex syndrome associated with exhaustion not relieved by sleep. It occurs frequently in older adults in the context of chronic disease and is associated with decreased physical capacity. Whether a mitochondrial dysfunction and therefore an impaired energy production might contribute to the development of fatigue during aging is yet unknown. The aim of this study was to evaluate mitochondrial respiration of peripheral blood mononuclear cells (PBMCs) in older patients with and without fatigue.
Fatigue was determined according to the Brief Fatigue Inventory. Mitochondrial respiration of freshly isolated PBMCs was investigated by high-resolution respirometry using the Oroboros Oxygraph-O2k. Functional impairment and depressive symptoms were assessed using questionnaires.
23 geriatric patients (77.8 ± 4.9 years; 43.5% female) with fatigue and 22 without fatigue (75.4 ± 5.4 years; 45.5% female) were analyzed. Patients with fatigue exhibited more functional limitations and more depressive symptoms. High-resolution respirometry of intact PBMCs revealed a lower routine (4.82 ± 1.14 pmol/s versus 5.89 ± 1.90 pmol/s, p = 0.041) and maximum (6.55 ± 1.51 pmol/s versus 8.43 ± 3.67 pmol/s, p = 0.013) oxygen consumption rate, resulting in a reduced ATP-linked respiration (4.26 ± 1.00 pmol/s versus 5.09 ± 1.53 pmol/s, p = 0.035) of PBMCs from geriatric patients with fatigue compared to controls without.
This short report shows that in this group of older patients, fatigue is associated with lower PBMC mitochondrial respiration. Whether the impaired mitochondrial respiration is accompanied by a reduced mitochondrial activity in other organs (e.g. muscle) remains to be elucidated.
疲劳是一种与睡眠无法缓解的疲惫相关的复杂综合征。它在患有慢性疾病的老年人中经常发生，并与身体能力下降有关。线粒体功能障碍是否会导致能量产生受损，从而导致衰老过程中疲劳的发展，目前尚不清楚。本研究旨在评估有和无疲劳的老年患者外周血单核细胞（PBMC）的线粒体呼吸。
根据简明疲劳量表确定疲劳。使用 Oroboros Oxygraph-O2k 通过高分辨率呼吸计研究新鲜分离的 PBMC 的线粒体呼吸。使用问卷评估功能障碍和抑郁症状。
分析了 23 名患有疲劳的老年患者（77.8±4.9 岁；43.5%为女性）和 22 名无疲劳的患者（75.4±5.4 岁；45.5%为女性）。有疲劳的患者表现出更多的功能障碍和更多的抑郁症状。完整 PBMC 的高分辨率呼吸测量显示，常规呼吸（4.82±1.14 pmol/s 与 5.89±1.90 pmol/s，p=0.041）和最大呼吸（6.55±1.51 pmol/s 与 8.43±3.67 pmol/s，p=0.013）氧消耗率降低，导致 PBMC 的 ATP 连接呼吸减少（4.26±1.00 pmol/s 与 5.09±1.53 pmol/s，p=0.035）疲劳的老年患者与对照组相比。
本报告显示，在这组老年患者中，疲劳与 PBMC 线粒体呼吸降低有关。受损的线粒体呼吸是否伴有其他器官（如肌肉）的线粒体活性降低，仍有待阐明。
