Turko Nir A, Shaked Natan T
Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv 69978, Israel.
Biomed Opt Express. 2020 Oct 22;11(11):6649-6658. doi: 10.1364/BOE.404368. eCollection 2020 Nov 1.
We report a cross-talk free simultaneous three-wavelength digital holographic microscopy setup for spectroscopic imaging of biological cells during flow. The feasibility of the proposed measurement technique is demonstrated on erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) molecule absorption on wavelength. From the spectroscopic quantitative phase profiles of cells acquired during flow in a microfluidic device, we decoupled the refractive index and the physical thickness. We then used our quantitative phase imaging results to dynamically calculate the mean cell volume (MCV), mean corpuscular Hb concentration (MCHC), mean corpuscular Hb content (MCH) and sphericity index.
我们报告了一种用于流动状态下生物细胞光谱成像的无串扰同步三波长数字全息显微镜装置。由于红细胞独特的形态以及血红蛋白(Hb)分子吸收对波长的依赖性,我们在红细胞上验证了所提出测量技术的可行性。从在微流控装置中流动的细胞的光谱定量相位分布图中,我们解耦了折射率和物理厚度。然后,我们利用定量相位成像结果动态计算平均细胞体积(MCV)、平均红细胞血红蛋白浓度(MCHC)、平均红细胞血红蛋白含量(MCH)和球形指数。