Belayneh Yaschilal Muche, Birru Eshetie Melese, Ambikar Digambar
Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
J Exp Pharmacol. 2019 Jul 25;11:73-83. doi: 10.2147/JEP.S212206. eCollection 2019.
Diabetes mellitus is one of the most common chronic health problems in the world. As currently available antidiabetic medications have limitations in terms of safety, efficacy, and cost, it is an important research area to investigate medicinal plants for new antidiabetic compounds that can lead to effective, safe and less costly pharmacotherapy. The present study was done to evaluate the antidiabetic and antidyslipidemic activities of 80% methanolic seed extract of (Ait.) Benth. (Fabaceae) in mice.
Blood glucose lowering activity of three doses (2.75 mg/kg, 5.5 mg/kg and 11 mg/kg) of the hydromethanolic seed extract of was studied in three animal models: normoglycemic mice, oral glucose-loaded mice, and streptozotocin-induced diabetic mice. Additionally, the effect of the seed extract on body weight and serum lipid profile was studied in the streptozotocin-induced diabetic mice. Glibenclamide (5 mg/kg) was used as a standard drug in all animal models of the study. Blood glucose level was measured using a glucose meter, whereas serum lipid level was measured using an automated chemistry analyzer. Data were analyzed using one-way analysis of variance followed by Tukey's post hoc multiple comparison test.
Hydromethanolic extract of seeds showed blood glucose lowering activity in all animal models, and it improved body weight loss and diabetic dyslipidemia in diabetic mice after 14 days of treatment.
This study revealed that hydromethanolic extract of seeds has significant hypoglycemic, antihyperglycemic and antihyperlipidemic activities.
糖尿病是世界上最常见的慢性健康问题之一。由于目前可用的抗糖尿病药物在安全性、有效性和成本方面存在局限性，因此研究药用植物以寻找可导致有效、安全且成本较低的药物治疗的新型抗糖尿病化合物是一个重要的研究领域。本研究旨在评估多花黄精（Ait.）Benth.（豆科）种子80%甲醇提取物对小鼠的抗糖尿病和抗血脂异常活性。
在三种动物模型中研究了多花黄精种子水甲醇提取物三个剂量（2.75mg/kg、5.5mg/kg和11mg/kg）的降血糖活性：正常血糖小鼠、口服葡萄糖负荷小鼠和链脲佐菌素诱导的糖尿病小鼠。此外，在链脲佐菌素诱导的糖尿病小鼠中研究了种子提取物对体重和血清脂质谱的影响。在本研究的所有动物模型中，格列本脲（5mg/kg）用作标准药物。使用血糖仪测量血糖水平，而使用自动化学分析仪测量血清脂质水平。数据采用单因素方差分析，随后进行Tukey事后多重比较检验进行分析。
多花黄精种子水甲醇提取物在所有动物模型中均显示出降血糖活性，并且在治疗14天后改善了糖尿病小鼠的体重减轻和糖尿病血脂异常。
本研究表明，多花黄精种子水甲醇提取物具有显著的降血糖、抗高血糖和抗高血脂活性。
