Sitati Concepta N W, Ogila Kenneth O, Waihenya Rebecca W, Ochola Lucy A
Department of Biological Sciences, School of Pure and Applied Sciences, Mount Kenya University, P.O. Box 1869-30200, Kitale, Kenya.
Zoology Department, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
Evid Based Complement Alternat Med. 2021 Oct 19;2021:3025848. doi: 10.1155/2021/3025848. eCollection 2021.
The mushroom is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of extracts on certain bacterial strains that included and representing the Gram-negative bacteria and and representing Gram-positive bacteria. The fungi were represented by and . The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against . However, both methanol and aqueous extracts did not inhibit growth of . In conclusion, extracts are a promising source of novel antimicrobial and antifungal agents.
该蘑菇是一种野生可食用蘑菇，属于白蘑科。过去几十年的研究表明，蘑菇及其活性成分对多种生物系统具有有益作用。一些蘑菇确实具有抗菌特性。对该蘑菇进行了定性植物化学分析，以确定具有重要生物活性的化合物的存在。本研究还调查了该蘑菇提取物对某些细菌菌株的影响，其中包括代表革兰氏阴性菌的大肠杆菌和肺炎克雷伯菌，以及代表革兰氏阳性菌的金黄色葡萄球菌和枯草芽孢杆菌。真菌以酿酒酵母和白色念珠菌为代表。该蘑菇采集于肯尼亚西部，风干后粉碎成粉末，然后分别用水、甲醇和二氯甲烷（DCM）溶剂进行提取。采用纸片扩散法评估抗菌和抗真菌活性。对水提取物进行的定性植物化学筛选显示存在生物碱、黄酮类化合物、甾体、甾醇、皂苷、酚类、碳水化合物和蛋白质。这三种提取物对受试细菌菌株均表现出抗菌活性。在受试细菌菌株中，DCM提取物显示出更高的效果。这三种提取物对白色念珠菌均显示出抗真菌作用。然而，甲醇提取物和水提取物均未抑制酿酒酵母的生长。总之，该蘑菇提取物是新型抗菌和抗真菌剂的一个有前景的来源。
