Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt; Health Sciences Division, Veterinary Sciences Program, Al Ain Men's Campus, Higher Colleges of Technology, Al Ain 17155, United Arab Emirates.
Comp Immunol Microbiol Infect Dis. 2021 Feb;74:101576. doi: 10.1016/j.cimid.2020.101576. Epub 2020 Nov 30.
Canine parvovirus type 2 (CPV-2) causes a highly contagious gastroenteritis disease of dogs and wild canids. To investigate the CPV-2 prevalence in Dakahlia Governorate, Egypt, a total of 50 fecal swabs were collected from suspected diseased dogs during 2016-2017. Out of 50 collected samples, 35 samples (70 %) presented positive results for CPV-2 using immuno-chromatography (IC) as a rapid test. CPV-2DNA was detected in 42 samples (84 %) by using polymerase chain reaction (PCR). The frequencies of CPV-2 were significantly higher in German shepherd breed (46 %; 23/50) and in age groups less than 6 months (76%; 38/50). We evaluated the breed, age, sex, rapid test results and clinical signs as predictors for classification of animal status into infected and not infected. The best predictors for classification process were rapid test result and clinical signs. Both CPV-2b and CPV-2c subtypes were detected by CPV2-VP2 gene sequences analysis. Deduced amino acid sequences alignment showed substitutions at 3 sites (Arg453Pro, Ala574Glu and Gln457Leu). Further investigations are needed to reveal the genetic and antigenic relation between field and vaccinal strains of CPV-2 in Egypt.
犬细小病毒 2 型（CPV-2）可引起犬和野生犬科动物高度传染性的胃肠炎疾病。为了调查埃及达卡利亚省的 CPV-2 流行情况，在 2016-2017 年间共从疑似患病犬中采集了 50 份粪便拭子。在收集的 50 个样本中，35 个样本（70%）通过免疫层析法（IC）作为快速检测呈 CPV-2 阳性。通过聚合酶链反应（PCR）检测到 42 个样本（84%）中存在 CPV-2DNA。CPV-2 的频率在德国牧羊犬品种（46%；23/50）和 6 个月以下年龄组（76%；38/50）中显着更高。我们评估了品种、年龄、性别、快速检测结果和临床症状作为将动物状态分类为感染和未感染的预测因子。分类过程的最佳预测因子是快速检测结果和临床症状。通过 CPV2-VP2 基因序列分析检测到 CPV-2b 和 CPV-2c 亚型。推导的氨基酸序列比对显示在 3 个位点发生取代（Arg453Pro、Ala574Glu 和 Gln457Leu）。需要进一步研究以揭示埃及 CPV-2 田间和疫苗株之间的遗传和抗原关系。
