Ndiana Linda A, Lanave Gianvito, Zarea Aya A K, Desario Costantina, Odigie Eugene A, Ehab Fouad A, Capozza Paolo, Greco Grazia, Buonavoglia Canio, Decaro Nicola
Department of Veterinary Medicine, University of Bari, Bari, Italy.
Department of Veterinary Microbiology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Nigeria.
Front Vet Sci. 2022 Jul 22;9:932247. doi: 10.3389/fvets.2022.932247. eCollection 2022.
Canine parvovirus (CPV) and feline panleukopenia virus (FPV), now included in the unique species (CPPV1), have been circulating in dogs and cats for several decades and are considered the causes of clinically important diseases, especially in young animals. While genetic evidence of the circulation of parvoviruses in Egyptian domestic carnivores has been provided since 2016, to date, all available data are based on partial fragments of the VP2 gene. This study reports the molecular characterization of CPPV strains from Egypt based on the full VP2 gene. Overall, 196 blood samples were collected from dogs and cats presented at veterinary clinics for routine medical assessment in 2019 in Egypt. DNA extracts were screened and characterized by real-time PCR. Positive samples were amplified by conventional PCR and then were sequenced. Nucleotide and amino acid changes in the sequences were investigated and phylogeny was inferred. DNA was detected in 18 out of 96 dogs (18.8%) and 7 of 100 cats (7%). Phylogenetic analyses based on the full VP2 gene revealed that 9 sequenced strains clustered with different CPV clades (5 with 2c, 2 with 2a, 1 with 2b, and 1 with 2) and 1 strain with the FPV clade. All three CPV variants were detected in dog and cat populations with a predominance of CPV-2c strains (7 of 18, 38.9%) in dog samples, thus mirroring the circulation of this variant in African, European, and Asian countries. Deduced amino acid sequence alignment revealed the presence of the previously unreported unique mutations S542L, H543Q, Q549H, and N557T in the Egyptian CPV-2c strains.
犬细小病毒（CPV）和猫泛白细胞减少症病毒（FPV），现归为同一物种（CPPV1），已在犬猫中传播数十年，被认为是重要临床疾病的病因，尤其是在幼龄动物中。虽然自2016年以来已有埃及家养食肉动物中细小病毒传播的基因证据，但迄今为止，所有可用数据均基于VP2基因的部分片段。本研究报告了基于完整VP2基因的埃及CPPV毒株的分子特征。总体而言，2019年在埃及的兽医诊所收集了196份犬猫血液样本，用于常规医学评估。通过实时PCR对DNA提取物进行筛选和鉴定。阳性样本通过常规PCR扩增，然后进行测序。研究了序列中的核苷酸和氨基酸变化，并推断了系统发育。在96只犬中的18只（18.8%）和100只猫中的7只（7%）检测到DNA。基于完整VP2基因的系统发育分析表明，9个测序毒株与不同的CPV分支聚类（5个与2c聚类，2个与2a聚类，1个与2b聚类，1个与2聚类），1个毒株与FPV分支聚类。在犬猫群体中检测到了所有三种CPV变体，犬样本中以CPV-2c毒株为主（18只中的7只，38.9%），这反映了该变体在非洲、欧洲和亚洲国家的传播情况。推导的氨基酸序列比对显示，埃及CPV-2c毒株中存在先前未报道的独特突变S542L、H543Q、Q549H和N557T。
