Prasetyaningtyas Neken, Jatiatmaja Nabilah Azzahra, Radithia Desiana, Hendarti Hening Tuti, Parmadiati Adiastuti Endah, Hadi Priyo, Mahdani Fatma Yasmin, Ernawati Diah Savitri, Zain Rosnah Binti, Ayuningtyas Nurina Febriyanti
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eur J Dent. 2021 May;15(2):320-324. doi: 10.1055/s-0040-1721312. Epub 2020 Dec 7.
The aim of this study is to analyze the tongue epithelial response to cigarette smoke exposure on a number of macrophages, lymphocytes, plasma cells, and matrix metalloproteinase 9 (MMP-9) expression to determine the risk factor of oral cancer development.
Thirty will be exposed to two kinds of cigarette smoke by a smoking pump for 4 and 8 weeks. The tongues were collected to analyze the number of macrophages, lymphocytes, and plasma cells with hematoxylin-eosin. The MMP-9 expression was similarly analyzed with immunohistochemical staining and then compared with the control group.
The number of macrophages, lymphocytes, and MMP-9 expression was higher in the 8-week cigarette smoke exposure compared to the 4-week cigarette smoke exposure and the control group ( < 0.000). The number of plasma cell did not differ in the 8-week cigarette smoke exposure from that of the control group ( > 0.05). The number of plasma cells in the tongue tissue during the 4-week cigarette smoke exposure was not determined.
Cigarette smoke exposure induces the risk of oral cancer development as a result of an increase in the number of macrophages, lymphocytes, and MMP-9 expression in the tongue epithelial.
本研究旨在分析舌上皮对香烟烟雾暴露的反应，涉及巨噬细胞、淋巴细胞、浆细胞数量以及基质金属蛋白酶9（MMP-9）的表达，以确定口腔癌发生的危险因素。
30只（此处原文“Thirty ”后缺少具体实验对象，推测为某种动物）将通过吸烟泵暴露于两种香烟烟雾中，持续4周和8周。采集舌头，用苏木精-伊红染色分析巨噬细胞、淋巴细胞和浆细胞的数量。用免疫组织化学染色同样分析MMP-9的表达，然后与对照组进行比较。
与4周香烟烟雾暴露组和对照组相比，8周香烟烟雾暴露组的巨噬细胞、淋巴细胞数量及MMP-9表达更高（<0.000）。8周香烟烟雾暴露组的浆细胞数量与对照组无差异（>0.05）。未测定4周香烟烟雾暴露期间舌组织中的浆细胞数量。
香烟烟雾暴露由于舌上皮中巨噬细胞、淋巴细胞数量增加及MMP-9表达升高，诱导了口腔癌发生的风险。
