Leoni Claudia, Piancone Elisabetta, Sasanelli Nicola, Bruno Giovanni Luigi, Manzari Caterina, Pesole Graziano, Ceci Luigi R, Volpicella Mariateresa
Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, CNR, Via Amendola 165/A, 70126 Bari, Italy.
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy.
Microorganisms. 2020 Dec 3;8(12):1922. doi: 10.3390/microorganisms8121922.
The artificial introduction in the soil of antagonistic microorganisms can be a successful strategy, alternative to agrochemicals, for the control of the root-knot nematodes ( spp.) and for preserving plant health. On the other hand, plant roots and the associated rhizosphere constitute a complex system in which the contribution of microbial community is fundamental to plant health and development, since microbes may convert organic and inorganic substances into available plant nutrients. In the present study, the potential nematicidal activity of the biopesticide ( strain MX-95) against the root-knot nematode in infected tomato plants was investigated. Specifically, the effect of the treatment on plant fitness was evaluated observing the plant morphological traits and also considering the nematode propagation parameters, the MX-95 vitality and population density. In addition, the treatment effects on the rhizosphere microbiome were analysed by a metabarcoding procedure. Treatments with isolate MX-95 significantly decreased root gall severity index and soil nematode population. The treatment also resulted in increased rhizosphere microbial populations. can be favourably considered as a new bionematicide to control infestation.
在土壤中人工引入拮抗微生物可能是一种成功的策略，可替代农用化学品来防治根结线虫并保护植物健康。另一方面，植物根系及其相关的根际构成了一个复杂的系统，其中微生物群落的作用对于植物健康和发育至关重要，因为微生物可以将有机和无机物质转化为植物可利用的养分。在本研究中，研究了生物农药（菌株MX - 95）对受感染番茄植株中根结线虫的潜在杀线虫活性。具体而言，通过观察植物形态特征并考虑线虫繁殖参数、MX - 95活力和种群密度，评估了该处理对植物适应性的影响。此外，通过元条形码程序分析了该处理对根际微生物组的影响。用分离株MX - 95处理显著降低了根瘤严重指数和土壤线虫种群数量。该处理还导致根际微生物种群增加。可以将其视为一种防治侵染的新型生物杀线虫剂。
