College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Biomolecules. 2020 Dec 3;10(12):1635. doi: 10.3390/biom10121635.
L. is highly adaptable and resistant to a range of abiotic stressors, allowing its growth in various environments. However, it is unclear how responds to common environmental stresses. We explored the physiological, transcriptomic, and metabolic responses of to short-term drought, salt, and heat stresses. Proline, HO, and ABA contents, along with CAT activity, increased under all three types of stress. SOD activity increased under salt and heat stresses, while soluble protein and IAA contents decreased under drought and salt stresses. With respect to metabolites, D-glyceric acid increased in response to drought and salt stresses, whereas isomaltose 1, oxalamide, and threonine 2 increased under drought. Piceatannol 2,4-hydroxybutyrate and 1,3-diaminopropane increased under salt stress, whereas 4-aminobutyric acid 1 and galactonic acid increased in response to heat stress. Genes regulating nitrogen assimilation were upregulated only under drought, while the GRAS gene was upregulated under all three types of stressors. ARF genes were downregulated under heat stress, whereas genes encoding HSF and SPL were upregulated. Additionally, we predicted that miR156, miR160, miR172, and their target genes participate in stress responses. Our study provides valuable data for studying the multilevel response to drought, salinity, and heat in .
L. 具有高度的适应性和抗逆性，可以在各种环境中生长。然而，目前尚不清楚 如何应对常见的环境压力。我们探讨了 对短期干旱、盐和热胁迫的生理、转录组和代谢反应。脯氨酸、HO 和 ABA 含量以及 CAT 活性在三种胁迫下均增加。SOD 活性在盐和热胁迫下增加，而干旱和盐胁迫下可溶性蛋白和 IAA 含量降低。就代谢物而言，D-甘油酸对干旱和盐胁迫有反应，而异麦芽糖 1、草酰亚胺和苏氨酸 2 在干旱下增加。盐胁迫下，皮考汀 2、4-羟基丁酸和 1,3-二氨基丙烷增加，而 4-氨基丁酸 1 和半乳糖酸对热胁迫有反应。仅在干旱下上调调节氮同化的基因，而 GRAS 基因在三种胁迫下均上调。ARF 基因在热胁迫下下调，而编码 HSF 和 SPL 的基因上调。此外，我们预测 miR156、miR160、miR172 及其靶基因参与应激反应。本研究为研究 在干旱、盐度和热胁迫下的多层次反应提供了有价值的数据。
