Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland.
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki Str.1, 20-093 Lublin, Poland.
Molecules. 2018 Sep 6;23(9):2277. doi: 10.3390/molecules23092277.
Lungwort ( L., Boraginaceae) is considered to possess therapeutic properties and it has been traditionally used as a remedy against various lung disorders in many countries. Nevertheless, very few data concerning its phytochemical composition are available. This research aims to provide a detailed description of specialized metabolites from the aerial parts of lungwort. Nine previously undescribed and 36 known phenolic compounds were detected in the 50% methanolic extract. Following multistep preparative procedures, structures of newly discovered compounds were determined using one- and two-dimensional techniques of NMR spectroscopy. Among the identified compounds were caffeic acid esters with aliphatic hydroxycarboxylic acids, conjugates of dicaffeic acid with rosmarinic acid, and previously unknown isomers of isosalvianolic acid A and yunnaneic acid E, as well as other lignans. Concentrations of all identified phenolic derivatives in the investigated herbal material were estimated using a method based on liquid chromatography with high-resolution mass spectrometry detection. Seasonal changes in the concentration of metabolites were also investigated using targeted and untargeted metabolomics techniques.
肺草（L.，紫草科）被认为具有治疗特性，在许多国家传统上被用作治疗各种肺部疾病的药物。然而，关于其植物化学成分的资料非常有限。本研究旨在详细描述肺草地上部分的特殊代谢物。在 50%甲醇提取物中检测到 9 种以前未描述的和 36 种已知的酚类化合物。通过多步制备程序，使用 NMR 光谱的一维和二维技术确定了新发现化合物的结构。鉴定出的化合物包括具有脂族羟基羧酸的咖啡酸酯、迷迭香酸与二咖啡酸的轭合物，以及以前未知的异萨尔维安酸 A 和云南酸 E 的异构体，以及其他木脂素。使用基于高效液相色谱-高分辨率质谱检测的方法估计了所研究草药材料中所有鉴定出的酚类衍生物的浓度。还使用靶向和非靶向代谢组学技术研究了代谢物浓度的季节性变化。
