Institute of Burns, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan 430060, PR China.
Institute of Burns, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan 430060, PR China.
Burns. 2021 Feb;47(1):133-139. doi: 10.1016/j.burns.2020.10.016. Epub 2020 Nov 7.
Impaired wound healing, which is due to various external and internal factors that are involved in wound pathophysiology, leads to high rates of morbidity and mortality worldwide. Oxidative stress injury is an important factor that affects wound healing by changing the whole healing process. So, resveratrol, a dietary fruits polyphenol, which is known for its antioxidant properties, maybe the candidate to accelerate the wound-healing process.
The Human Umbilical Vein Endothelial Cells (HUVECs) was used for in vitro experiments to evaluate the effect of resveratrol on hyperglycemia-induced gene expression, oxidative stress and cell proliferation. The diabetic rat model was used to evaluate the effect of resveratrol on cutaneous burn injury healing process.
Increases in HO decreased cell viability with the 0-800 μM concentration range, and resveratrol could protect HUVECs against HO-induced injury. The scratched wound closed rate in HO group was significantly smaller than the Control group (p < 0.05) and Resveratrol + HO group (p < 0.05). The fluorescence intensity of ROS was lower in Control and Resveratrol + HO groups than HO group. Correspondingly, compared to HO group, the expressions of Mn-SOD and nuclear Nrf2 (N-Nrf2) was up-regulated in Resveratrol + HO group (p < 0.05). In vivo, compared with the saline group, using resveratrol could significantly accelerate wound healing of rats on Day 14 (p < 0.05) and make the regenerated skin structure more complete and inflammatory response lower. Moreover, the expressions of Mn-SOD was significantly up-regulated after using resveratrol.
Resveratrol has the positive effects on promoting the acceleration and quality of skin wound healing, which maybe at least in part caused by the up-regulation of nuclear Nrf2 and Mn-SOD that subsequently attenuated oxidative stress.
由于各种涉及伤口病理生理学的外部和内部因素，伤口愈合受损导致全球发病率和死亡率居高不下。氧化应激损伤是影响伤口愈合的一个重要因素，它通过改变整个愈合过程来实现。因此，白藜芦醇，一种已知具有抗氧化特性的膳食水果多酚，可能是加速伤口愈合过程的候选物质。
使用人脐静脉内皮细胞（HUVEC）进行体外实验，以评估白藜芦醇对高血糖诱导的基因表达、氧化应激和细胞增殖的影响。使用糖尿病大鼠模型评估白藜芦醇对皮肤烧伤愈合过程的影响。
在 0-800μM 浓度范围内，HO 的增加降低了细胞活力，而白藜芦醇可以保护 HUVEC 免受 HO 诱导的损伤。划痕伤口闭合率在 HO 组明显小于对照组（p<0.05）和白藜芦醇+HO 组（p<0.05）。与 HO 组相比，Control 和 Resveratrol+HO 组的 ROS 荧光强度较低。相应地，与 HO 组相比，Resveratrol+HO 组 Mn-SOD 和核 Nrf2（N-Nrf2）的表达上调（p<0.05）。在体内，与生理盐水组相比，使用白藜芦醇可显著加速大鼠第 14 天的伤口愈合（p<0.05），并使再生皮肤结构更加完整，炎症反应更低。此外，使用白藜芦醇后 Mn-SOD 的表达明显上调。
白藜芦醇对促进皮肤伤口愈合的加速和质量有积极作用，这至少部分归因于核 Nrf2 和 Mn-SOD 的上调，从而减轻氧化应激。
