Kaleci Belisa, Koyuturk Meral
Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Dermatol Ther. 2020 Nov;33(6):e14357. doi: 10.1111/dth.14357. Epub 2020 Oct 10.
Oxidative stress (OS) is implicated in the pathogenesis of diabetic, chronic, and inflamed wounds. In these cases, wound healing is difficult and remains a big clinical challenge. As fibroblasts are crucial in wound healing by producing extracellular matrix and wound contraction, the researchers investigated the resveratrol (RES) effects as a potential therapeutic alternative, on an in vitro OS and wound healing model established by 3T3 Swiss Albino fibroblasts. OS was induced by H O , and 1-100 μM RES doses were applied on cells for up to 48 hours. The cell proliferation was assessed by the population doubling time and bromodeoxyuridine immunocytochemistry. The OS levels were determined by fluorescence staining and all values were calculated by the ImageJ program. The collagen-I expression (COL1A2 mRNA) was evaluated by a real-time PCR. Cell migration was evaluated by wound closure assay and the ultrastructure was evaluated by electron microscopy. The cell proliferation index significantly decreased in H O incubation and increased with RES application. RES application reduced OS levels and stabilized cell proliferation, but no differences were found in COL1A2 mRNA expression. RES-treated cells showed better proliferation, migration rates, and ultrastructural preservation. RES administration decreases OS levels and improves the healing process by increasing cell proliferation and migration quality, suggesting it is a powerful candidate for the treatment of skin wounds.
氧化应激（OS）与糖尿病性、慢性和炎症性伤口的发病机制有关。在这些情况下，伤口愈合困难，仍然是一个重大的临床挑战。由于成纤维细胞通过产生细胞外基质和伤口收缩在伤口愈合中起关键作用，研究人员在由3T3瑞士白化病成纤维细胞建立的体外氧化应激和伤口愈合模型中，研究了白藜芦醇（RES）作为一种潜在治疗选择的作用。通过过氧化氢诱导氧化应激，并将1-100μM的白藜芦醇剂量应用于细胞长达48小时。通过群体倍增时间和溴脱氧尿苷免疫细胞化学评估细胞增殖。通过荧光染色测定氧化应激水平，所有值均通过ImageJ程序计算。通过实时聚合酶链反应评估I型胶原蛋白表达（COL1A2 mRNA）。通过伤口闭合试验评估细胞迁移，并通过电子显微镜评估超微结构。在过氧化氢孵育中细胞增殖指数显著降低，而随着白藜芦醇的应用而增加。白藜芦醇的应用降低了氧化应激水平并稳定了细胞增殖，但在COL1A2 mRNA表达中未发现差异。经白藜芦醇处理的细胞表现出更好的增殖、迁移率和超微结构保存。白藜芦醇给药降低了氧化应激水平，并通过提高细胞增殖和迁移质量改善了愈合过程，表明它是治疗皮肤伤口的有力候选药物。
