Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan
Anticancer Res. 2020 Dec;40(12):6713-6721. doi: 10.21873/anticanres.14694.
BACKGROUND/AIM: Although our understanding of the molecular mechanisms of gastric cancer (GC) development and progression is steadily deepening, the clinical outcome of GC patients remains inadequate. The identification of molecules associated with GC will help improve prognosis. We aimed to identify the molecules involved in GC progression and metastasis.
Transcriptome analysis was performed on surgically resected gastric tissue from patients with hepatic metastasis. Fourteen cell lines and 230 pairs of primary GC tissues and their corresponding normal adjacent tissues were included in the mRNA expression analysis.
Adhesion molecule with Ig like domain 2 (AMIGO2) was identified as a gene of interest. The levels of AMIGO2 mRNA positively correlated with those encoding FOXC2, NODAL, GEMIN2 and negatively correlated with TFPI2. Patients with high AMIGO2 expression experienced significantly shorter disease-free survival and overall survival. High levels of AMIGO2 were associated with poor prognosis.
Patients with GC with high AMIGO2 mRNA levels experienced significantly shorter survival, suggesting that AMIGO2 may serve as a prognostic biomarker for GC.
背景/目的：尽管我们对胃癌（GC）发展和转移的分子机制的理解在不断深入，但 GC 患者的临床结局仍然不理想。鉴定与 GC 相关的分子有助于改善预后。本研究旨在鉴定与 GC 进展和转移相关的分子。
对伴有肝转移的手术切除胃组织进行转录组分析。在 mRNA 表达分析中纳入了 14 种细胞系和 230 对原发性 GC 组织及其相应的正常邻近组织。
鉴定出黏附分子 Ig 样结构域 2（AMIGO2）为感兴趣的基因。AMIGO2 mRNA 水平与编码 FOXC2、NODAL、GEMIN2 的水平呈正相关，与 TFPI2 的水平呈负相关。AMIGO2 高表达的患者无病生存和总生存时间显著缩短。高 AMIGO2 水平与预后不良相关。
GC 患者中 AMIGO2 mRNA 水平较高者的生存时间明显缩短，提示 AMIGO2 可能是 GC 的预后生物标志物。
