Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, King Salman International University, South Sinai, Egypt.
J Sci Food Agric. 2021 Jun;101(8):3429-3438. doi: 10.1002/jsfa.10973. Epub 2020 Dec 18.
Ginger rhizome (Zingiber officinale) is a well-known spice and medicinal plant that is widely used in the Egyptian market as a spice, flavor and medicinal herb for different diseases. Since it is not cultivated as rhizomes in Egypt, ginger is imported from other countries, which may impact its quality. In this study, UV spectroscopy and high-performance liquid chromatography (HPLC) were applied as efficient available techniques for the discrimination and quality control of ginger collected from different geographical origins in combination with chemometrics. In addition, HPLC was applied to investigate the stability of ginger samples upon storage for 3 years to trace the changes in their main active constituents.
Data obtained from both UV and HPLC in combination with Principal Component Analysis (PCA) displayed proper discrimination of the samples according to their geographical origins. Regarding the stability study, ginger samples showed a significant decrease in quality after storage for 3 years, in which significant variation in the main pungent principles (6-, 8-, 10-gingerols and 6-shogaol) were observed. PCA failed to discriminate between ginger samples after long-time storage, so the applied model could discriminate between ginger samples before and after storage.
UV and HPLC in combination with chemometrics can be applied as a successful tool for the study of quality, stability and geographical discrimination of ginger. © 2020 Society of Chemical Industry.
姜根茎(Zingiber officinale)是一种广受欢迎的香料和药用植物,在埃及市场上被广泛用作香料、调味品和治疗各种疾病的草药。由于埃及不种植姜根茎,因此姜是从其他国家进口的,这可能会影响其质量。本研究采用紫外光谱法和高效液相色谱法(HPLC)结合化学计量学,对不同地理来源的姜进行鉴别和质量控制。此外,还应用 HPLC 研究了姜样品在储存 3 年内的稳定性,以追踪其主要活性成分的变化。
紫外和 HPLC 结合主成分分析(PCA)的数据显示,样品根据其地理来源得到了适当的区分。关于稳定性研究,姜样品在储存 3 年后质量明显下降,其中主要辛辣成分(6-、8-、10-姜酚和 6-姜烯醇)发生了显著变化。PCA 未能在长时间储存后区分姜样品,因此所应用的模型可以区分储存前后的姜样品。
紫外和 HPLC 结合化学计量学可用于研究姜的质量、稳定性和地理差异。© 2020 英国化学学会。