Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia, Spain.
Biotechnology Application and Research Center, and Department of Molecular Biology, Faculty of Science and Letter, Bilecik Seyh Edebali University, Bilecik, Turkey.
Plant Cell Environ. 2021 Jul;44(7):2167-2184. doi: 10.1111/pce.13972. Epub 2020 Dec 22.
Plant reproduction is one key biological process that is very sensitive to heat stress and, as a result, enhanced global warming becomes a serious threat to agriculture. In this work, we have studied the effects of heat on germinated pollen of Arabidopsis thaliana both at the transcriptional and translational level. We have used a high-resolution ribosome profiling technology to provide a comprehensive study of the transcriptome and the translatome of germinated pollen at permissive and restrictive temperatures. We have found significant down-regulation of key membrane transporters required for pollen tube growth by heat, thus uncovering heat-sensitive targets. A subset of the heat-repressed transporters showed coordinated up-regulation with canonical heat-shock genes at permissive conditions. We also found specific regulations at the translational level and we have uncovered the presence of ribosomes on sequences annotated as non-coding. Our results demonstrate that heat impacts mostly on membrane transporters thus explaining the deleterious effects of heat stress on pollen growth. The specific regulations at the translational level and the presence of ribosomes on non-coding RNAs highlights novel regulatory aspects on plant fertilization.
植物繁殖是一个关键的生物学过程，对热应激非常敏感，因此，全球变暖加剧对农业构成了严重威胁。在这项工作中，我们研究了热对拟南芥萌发花粉在转录和翻译水平上的影响。我们使用高分辨率核糖体分析技术，对适宜和限制温度下萌发花粉的转录组和翻译组进行了全面研究。我们发现，热对花粉管生长所需的关键膜转运体有显著的下调作用，从而揭示了热敏感的靶标。一部分受抑制的转运体在适宜条件下与典型的热休克基因协同上调。我们还发现了翻译水平的特异性调控，并发现了核糖体在注释为非编码序列上的存在。我们的研究结果表明，热主要影响膜转运体，从而解释了热应激对花粉生长的有害影响。在翻译水平上的特异性调控和核糖体在非编码 RNA 上的存在，突出了植物受精过程中的新的调控方面。
