过表达 GmCDC25 与 GmCDC25 相互作用促进大豆株高。
Overexpression of promoted soybean height by interacting with GmCDC25.
机构信息
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Jilin Agricultural University , Changchun, Jilin, China (130118).
College of Tropical Crops, Hainan University , Haikou, Hainan, China (570228).
出版信息
Plant Signal Behav. 2021 Feb 1;16(2):1855017. doi: 10.1080/15592324.2020.1855017. Epub 2020 Dec 8.
GmGASA is the GASA gibberellin regulated cysteine-rich protein family. The expression of GmGASA is up-regulated by gibberellin, which is the longest plant hormone in plants playing vital roles in plant development. However, very few reports explaining the direct regulation of downstream genes by are available. In the current study, the , a member of the family affecting soybean height was identified. In the early stage, preliminary verification of the response of to gibberellin through phenotypic experiment was done. The promoter activity analysis confirmed that was induced by gibberellin. Subcellular localization showed that GmGASA32-GFP fusion protein enriched in the nucleus after gibberellin treatment. In order to confirm the function of GmGASA32 in the nucleus, we confirmed that the GASA domain in the C terminal of GmGASA32 can interact with GmCDC25 (cell cycle-associated protein) through the bimolecular fluorescence complementation (BiFC) assay.
GmGASA 是 GASA 赤霉素调节的富含半胱氨酸的蛋白质家族。GmGASA 的表达受赤霉素上调，赤霉素是植物中最长的植物激素，在植物发育中起着至关重要的作用。然而，很少有报道解释 对下游基因的直接调节。在本研究中，鉴定出一个影响大豆高度的 家族成员。在早期，通过表型实验对 的赤霉素反应进行了初步验证。启动子活性分析证实 受赤霉素诱导。亚细胞定位显示，GmGASA32-GFP 融合蛋白在赤霉素处理后富集在细胞核中。为了确认 GmGASA32 在核中的功能，我们通过双分子荧光互补（BiFC）实验证实 GmGASA32 中 C 端的 GASA 结构域可以与 GmCDC25（细胞周期相关蛋白）相互作用。
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