Kuznetsov Anatoliy, Bollin Colleen J
National Center for Biotechnology Information, U.S. National Library of Medicine, North Potomac, MD, USA.
Methods Mol Biol. 2021;2231:261-295. doi: 10.1007/978-1-0716-1036-7_16.
The book chapter introduces the National Center for Biotechnology Information (NCBI) Genome Workbench, a desktop GUI software package to manipulate and visualize complex molecular biology models provided in many data formats. Genome Workbench integrates graphical views and computational tools in a single package to facilitate discoveries. In this chapter we provide a step-by-step protocol guidance on how to do comparative analysis of sequences using NCBI BLAST and multiple sequence alignment algorithms, build phylogenetic trees, and use graphical views for sequences, alignments, and trees to validate the findings. The software package can be used to prepare high-quality whole genome submissions to NCBI. The software package is user-friendly and includes validation and editing tools to fix errors as part of preparing the submission.
本章介绍了国家生物技术信息中心（NCBI）基因组工作台，这是一个桌面GUI软件包，用于操作和可视化多种数据格式提供的复杂分子生物学模型。基因组工作台将图形视图和计算工具集成在一个软件包中，以促进发现。在本章中，我们提供了一个逐步的协议指南，介绍如何使用NCBI BLAST和多序列比对算法进行序列比较分析、构建系统发育树，以及使用序列、比对和树的图形视图来验证结果。该软件包可用于向NCBI提交高质量的全基因组数据。该软件包用户友好，包括验证和编辑工具，用于在准备提交数据时修复错误。
