Department of Hematology, The First People’s Hospital of Shangqiu, Shangqiu 476100, Henan, China.
Aging (Albany NY). 2020 Dec 3;13(1):1120-1131. doi: 10.18632/aging.202240.
Circular RNAs (circRNAs) are newly-discovered endogenous non-coding RNAs that have vital functions in regulating gene expression in tumorigenesis. Nonetheless, the function of circRNAs in acute myeloid leukemia (AML) are not yet clarified. In this analysis, hsa_circ_0079480, a novel circRNA, has been identified as being highly expressed in AML. Loss-of-function assays showed that reduction of hsa_circ_0079480 decreased the growth and stimulated apoptosis of AML cells . Furthermore, miR-654-3p was sponged by hsa_circ_0079480, and hepatoma-derived growth factor (HDGF) was targeted by miR-654-3p with respect to the fundamental mechanism. Moreover, the influence on growth and apoptosis of AML cells stimulated by hsa_circ_0079480 inhibition can be rescued by miR-654-3p inhibitor or HDGF overexpression. In summary, hsa_circ_0079480 is highly expressed in AML and drives by tumor progression via regulation of hsa_circ_0079480/miR-654-3p/HDGF axis, indicating that hsa_circ_0079480 may function as a new treatment target for AML therapy.
环形 RNA（circRNAs）是新发现的内源性非编码 RNA，在肿瘤发生中对基因表达具有重要的调控作用。然而，circRNAs 在急性髓系白血病（AML）中的功能尚不清楚。在本分析中，hsa_circ_0079480，一种新型 circRNA，被鉴定为在 AML 中高度表达。功能丧失实验表明，hsa_circ_0079480 的减少降低了 AML 细胞的生长并刺激了细胞凋亡。此外，hsa_circ_0079480 可以吸附 miR-654-3p，而 miR-654-3p 可以靶向肝癌衍生生长因子（HDGF），这是其基本机制。此外，hsa_circ_0079480 抑制对 AML 细胞生长和凋亡的影响可以通过 miR-654-3p 抑制剂或 HDGF 过表达来挽救。总之，hsa_circ_0079480 在 AML 中高度表达，并通过调节 hsa_circ_0079480/miR-654-3p/HDGF 轴驱动肿瘤进展，表明 hsa_circ_0079480 可能作为 AML 治疗的新治疗靶点。
