Papa Rosanna, Garzoli Stefania, Vrenna Gianluca, Sabatino Manuela, Sapienza Filippo, Relucenti Michela, Donfrancesco Orlando, Fiscarelli Ersilia Vita, Artini Marco, Selan Laura, Ragno Rino
Department of Public Health and Infectious Diseases, Sapienza University, p.le Aldo Moro 5, 00185 Rome, Italy.
Department of Drug Chemistry and Technology, Sapienza University, p.le Aldo Moro 5, 00185 Rome, Italy.
Int J Mol Sci. 2020 Dec 4;21(23):9258. doi: 10.3390/ijms21239258.
Bacterial biofilm plays a pivotal role in chronic () infection and its inhibition may represent an important strategy to develop novel therapeutic agents. The scientific community is continuously searching for natural and "green alternatives" to chemotherapeutic drugs, including essential oils (EOs), assuming the latter not able to select resistant strains, likely due to their multicomponent nature and, hence, multitarget action. Here it is reported the biofilm production modulation exerted by 61 EOs, also investigated for their antibacterial activity on strains, including reference and cystic fibrosis patients' isolated strains. The EOs biofilm modulation was assessed by Christensen method on five strains. Chemical composition, investigated by GC/MS analysis, of the tested EOs allowed a correlation between biofilm modulation potency and putative active components by means of machine learning algorithms application. Some EOs inhibited biofilm growth at 1.00% concentration, although lower concentrations revealed different biological profile. Experimental data led to select antibiofilm EOs based on their ability to inhibit biofilm growth, which were characterized for their ability to alter the biofilm organization by means of SEM studies.
细菌生物膜在慢性（）感染中起关键作用，抑制细菌生物膜可能是开发新型治疗药物的重要策略。科学界一直在不断寻找化疗药物的天然和“绿色替代品”，包括精油（EOs），认为精油不太可能产生耐药菌株，这可能是由于其多组分性质以及由此产生的多靶点作用。本文报道了61种精油对生物膜产生的调节作用，同时还研究了它们对包括参考菌株和囊性纤维化患者分离菌株在内的菌株的抗菌活性。通过克里斯滕森方法在五种菌株上评估了精油对生物膜的调节作用。通过气相色谱/质谱分析研究测试精油的化学成分，借助机器学习算法应用，使生物膜调节效力与推定的活性成分之间建立了相关性。一些精油在浓度为1.00%时抑制生物膜生长，尽管较低浓度显示出不同的生物学特性。实验数据促使根据其抑制生物膜生长的能力来选择抗生物膜精油，并通过扫描电子显微镜研究对其改变生物膜结构的能力进行了表征。
