Du Plessis J L, Malan L
Veterinary Research Institute, Onderstepoort.
Onderstepoort J Vet Res. 1987 Sep;54(3):319-25.
The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of studies on heartwater, including epidemiology, determination of the C. ruminantium infection rate of Amblyomma ticks and the evaluation of immunization against heartwater. The test can also be used to detect antibodies to the heartwater agent in the sera of game.
本文描述了抗原的制备、试剂的详细信息、抗种属缀合物的滴定以及间接荧光抗体试验的操作。记录了该试验的敏感性、特异性及其在检测反刍动物考德里氏体抗体方面的适用性。该试验具有高度的特异性和敏感性，可应用于广泛的心水病研究，包括流行病学研究、钝缘蜱考德里氏体感染率的测定以及心水病免疫接种效果的评估。该试验还可用于检测野生动物血清中的心水病病原体抗体。
