Anwar Khanda, Hussein Dlsoz, Salih Jamal
Microbiology Department, College of Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq.
Microbiology Department, Central Public Health Laboratory 1, Sulaymaniyah, Kurdistan Region, Iraq.
Int J Gen Med. 2020 Dec 2;13:1349-1357. doi: 10.2147/IJGM.S278574. eCollection 2020.
Diabetic foot infection (DFI) is a common and costly complication of diabetes that may be caused by various bacteria with multi-resistant genes. The aim of this study is to evaluate the efficacy of phenotypic methods for identification of methicillin-resistant (MRSA) with genotypic detection of MRSA-related genes.
In this cross-sectional study, swab samples were collected from patients with DFI from hospitals in Sulaimani/Iraq in April-July 2019. All the samples were processed for microbiological assessment and further MRSA phenotypic and genotypic testing.
A total of 46 swab samples were collected from diabetic foot ulcers of 29 males and 17 females. Most samples (93.5%) showed positive growth, with higher proportions of monomicrobial (23; 53.5%) than mixed-bacterial infections (20; 46.5%) and as the predominant pathogen. Conventional methods of MRSA detection, such as cefoxitin disc diffusion, can predict methicillin resistance in 45.8% of the cases. Real-time/conventional PCR showed that 41.6% of were positive for the gene, while none of the isolates was positive for .
was the predominant pathogen in DFI. Although cefoxitin and oxacillin disc diffusion methods can help in the prediction of MRSA, real-time PCR is a reliable method for MRSA detection and confirmation.
糖尿病足感染（DFI）是糖尿病常见且代价高昂的并发症，可能由多种具有多重耐药基因的细菌引起。本研究的目的是评估表型方法鉴定耐甲氧西林金黄色葡萄球菌（MRSA）与MRSA相关基因的基因型检测的效果。
在这项横断面研究中，于2019年4月至7月从伊拉克苏莱曼尼亚的医院中收集DFI患者的拭子样本。所有样本均进行微生物学评估以及进一步的MRSA表型和基因型检测。
共从29名男性和17名女性的糖尿病足溃疡中收集了46份拭子样本。大多数样本（93.5%）显示生长阳性，单一微生物感染（23例；53.5%）的比例高于混合细菌感染（20例；46.5%），且 作为主要病原体。MRSA检测的传统方法，如头孢西丁纸片扩散法，在45.8%的病例中可预测耐甲氧西林情况。实时/常规PCR显示，41.6%的 对 基因呈阳性，而所有分离株中无一例对 呈阳性。
在DFI中是主要病原体。虽然头孢西丁和苯唑西林纸片扩散法有助于预测MRSA，但实时PCR是检测和确认MRSA的可靠方法。
