Department of Microbiology, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran.
Department of Basic Medical Sciences, Faculty of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Curr Microbiol. 2024 Aug 3;81(9):295. doi: 10.1007/s00284-024-03633-9.
Staphylococcus aureus (S. aureus) is considered as one of the challenging ulcer infections in diabetic patients especially those who have acquired antibiotic-resistant infections. Nanotechnology products have enormous potential to treat diseases including infectious diseases. As chitosan and zinc oxide (ZnO) nanoparticles (NPs) have harbored a high antimicrobial effect, this survey was aimed to synthesize chitosan, ZnO, and ZnO-Urtica. diocia (ZnO-U. diocia) NPs, and to assess their antimicrobial effects and their influence on virulence genes expression in S. aureus isolates from diabetic ulcers. The antibacterial effect of NPs was detected by microdilution method. The most frequently components in U. diocia aqueous extract were linalool,4-thujanol, camphor, carvacrol, propanedioic acid, and di(butyl) phthalate. More than 95% of clinical S. aureus isolates were resistant to several antibiotics including erythromycin, cefoxitin, clindamycin, and ciprofloxacin. The most resistant isolates were S. aureus ATDS 52, ATDS 53, F5232, and F91. The lowest MIC and MBC by the NPs on the isolates was detected as 0.128 g/mL and 0.178 g/mL, respectively. A significant decrease of 90% in the expression rates of lukED and RNAIII genes was reported for S. aureus isolates treated with the NPs. The synthetized ZnO-U. diocia and chitosan NPs can be proposed as a reliable and effective antimicrobial agent targeting diabetic ulcers infections caused by S. aureus because of its high effects on the bacterial growth and virulence genes expression.
金黄色葡萄球菌（S. aureus）被认为是糖尿病患者中具有挑战性的溃疡感染之一，尤其是那些患有抗生素耐药感染的患者。纳米技术产品具有治疗疾病（包括传染病）的巨大潜力。由于壳聚糖和氧化锌（ZnO）纳米颗粒（NPs）具有很高的抗菌作用，本研究旨在合成壳聚糖、ZnO 和 ZnO-荨麻（ZnO-U. diocia）NPs，并评估它们对糖尿病溃疡中金黄色葡萄球菌分离株的抗菌作用及其对毒力基因表达的影响。通过微量稀释法检测 NPs 的抗菌效果。荨麻水提物中最常见的成分是芳樟醇、4-松油醇、樟脑、香芹酚、丙二酸和邻苯二甲酸二丁酯。超过 95%的临床金黄色葡萄球菌分离株对几种抗生素包括红霉素、头孢西丁、克林霉素和环丙沙星耐药。最耐药的分离株是 ATDS 52、ATDS 53、F5232 和 F91。 NPs 对分离株的最低 MIC 和 MBC 分别为 0.128 g/mL 和 0.178 g/mL。研究报告称， NPs 处理后的金黄色葡萄球菌分离株的 lukED 和 RNAIII 基因表达率显著下降了 90%。合成的 ZnO-U. diocia 和壳聚糖 NPs 可以作为一种针对金黄色葡萄球菌引起的糖尿病溃疡感染的可靠、有效的抗菌剂，因为它对细菌生长和毒力基因表达有很高的作用。
